Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
![Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)](https://www.abcam.com/ps/products/13/ab13496/Images/ab13496-20769-anti-alpha-b-crystallin-antibody-1b61-3g4-western-blot.jpg "Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)")
Key features and details
- Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Alpha B Crystallin antibody [1B6.1-3G4]
See all Alpha B Crystallin primary antibodies -
Description
Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Cow Alpha B Crystallin.
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Positive control
- Recombinant Bovine alpha B Crystallin Protein.
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General notes
This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituents: PBS, 0.00174% PMSF, 50% Glycerol -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
1B6.1-3G4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa). |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
May contribute to the transparency and refractive index of the lens. -
Tissue specificity
Lens as well as other tissues. -
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 1410 Human
- Omim: 123590 Human
- SwissProt: P02511 Human
- Unigene: 53454 Human
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Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
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Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : alpha Crystallin
Lane 3 : alpha A Crystallin
Lane 4 : alpha B Crystallin
Lane 5 : beta Crystallin
Predicted band size: 21 kDaThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)](https://www.abcam.com/ps/products/13/ab13496/Images/ab13496-277407-anti-alpha-b-crystallin-antibody-1b61-3g4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)Immunohistochemical analysis of human heart tissue using ab13496 at the concentration 10ug/ml.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)](https://www.abcam.com/ps/products/13/ab13496/Images/ab13496-20767-anti-alpha-b-crystallin-antibody-1b61-3g4-flow-cytometry.jpg)
Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)](https://www.abcam.com/ps/products/13/ab13496/Images/ab13496-277365-anti-alpha-b-crystallin-antibody-1b61-3g4-western-blot.jpg)
Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab13496 has been referenced in 40 publications.
- Ren Z et al. PNU282987 inhibits amyloid-ß aggregation by upregulating astrocytic endogenous aB-crystallin and HSP-70 via regulation of the a7AChR, PI3K/Akt/HSF-1 signaling axis. Mol Med Rep 22:201-208 (2020). PubMed: 32377707
- Andrews MG et al. mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex. Elife 9:N/A (2020). PubMed: 32876565
- Baburamani AA et al. Assessment of radial glia in the frontal lobe of fetuses with Down syndrome. Acta Neuropathol Commun 8:141 (2020). PubMed: 32819430
- Lornage X et al. Clinical, histological, and genetic characterization of PYROXD1-related myopathy. Acta Neuropathol Commun 7:138 (2019). PubMed: 31455395
- Pan B et al. Highly Dynamic Changes in the Activity and Regulation of Macroautophagy in Hearts Subjected to Increased Proteotoxic Stress. Front Physiol 10:758 (2019). PubMed: 31297061