Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

Lane 1 : Molecular weight marker
Lane 2 : alpha Crystallin

Lane 3 : alpha A Crystallin

Lane 4 : alpha B Crystallin
Lane 5 : beta Crystallin

Predicted band size: 21 kDa

Immunohistochemical analysis of murine skeletal muscle tissue, staining Alpha B Crystallin with ab13496. Sections were blocked for 1 hour in 1% BSA and 10% FCS. The primary antibody was conjugated to AlexaFluor®594 before diluting 1/20 and incubating overnight at 4°C.

Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

ab13496 staining Alpha B Crystallin in mouse brain tissue by Immunohistochemistry (free floating sections). Samples were incubated with the primary antibody at a 1/500 dilution for 16 hours at 4°C. A goat anti-mouse IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/10000 dilution.

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