Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

Reviews (4)Q&A (3)References (23)

Overview

  • Product name
    Anti-Alpha B Crystallin antibody [1B6.1-3G4]
    See all Alpha B Crystallin primary antibodies
  • Description
    Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
  • Host species
    Mouse
  • Specificity
    There is no reactivity with Beta-crystallin, AlphaA-crystallin, Hsp25, Hsp27 or Hsp47 proteins.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC, IP, Flow Cyt, IHC-FrFl, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Cow Alpha B Crystallin.

  • Positive control
    • Recombinant Bovine alpha B Crystallin Protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab13496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-FrFl 1/500.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    May contribute to the transparency and refractive index of the lens.
  • Tissue specificity
    Lens as well as other tissues.
  • Involvement in disease
    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similarities
    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Images

  • Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : alpha Crystallin

    Lane 3 : alpha A Crystallin

    Lane 4 : alpha B Crystallin
    Lane 5 : beta Crystallin

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

    Immunohistochemical analysis of human heart tissue using ab13496 at the concentration 10ug/ml.

  • Immunohistochemistry (Frozen sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Immunohistochemistry (Frozen sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)Image from Ravenscroft G et al., PLoS One. 2011;6(12):e28699. Epub 2011 Dec 9. Fig 7.; doi:10.1371/journal.pone.0028699; December 9, 2011, PLoS ONE 6(12): e28699.
    Immunohistochemical analysis of murine skeletal muscle tissue, staining Alpha B Crystallin with ab13496. Sections were blocked for 1 hour in 1% BSA and 10% FCS. The primary antibody was conjugated to AlexaFluor®594 before diluting 1/20 and incubating overnight at 4°C.
  • Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
  • Immunohistochemistry free floating - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Immunohistochemistry free floating - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)Image courtesy of Brian Hitt by Abreview
    ab13496 staining Alpha B Crystallin in mouse brain tissue by Immunohistochemistry (free floating sections). Samples were incubated with the primary antibody at a 1/500 dilution for 16 hours at 4°C. A goat anti-mouse IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/10000 dilution.

    See Abreview

References

This product has been referenced in:
  • San Gil R  et al. Using bicistronic constructs to evaluate the chaperone activities of heat shock proteins in cells. Sci Rep 7:2387 (2017). Read more (PubMed: 28539657) »
  • Kuipers HF  et al. Phosphorylation of aB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114:E1745-E1754 (2017). Read more (PubMed: 28196893) »
See all 23 Publications for this product

Publishing research using ab13496? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Rat Tissue sections (brain, 20 micron-thick free-floating sections)
Specification
brain, 20 micron-thick free-floating sections
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Dr. Marie-Caroline Cotel

Verified customer

Submitted May 28 2014

Immunoprecipitation abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (muscle)
Total protein in input
20 µg
Specification
muscle
Immuno-precipitation step
Protein G
Read More

Abcam user community

Verified customer

Submitted Apr 19 2012

Question

I would like to try the anti-alpha B Crystallin antibody (ab13496) for immunoprecipitation on mouse striated muscle.  

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Answer

DISCOUNT CODE: *** Expiration date: April 19th, 2012 I am very pleased to hear you would like to accept our offer and test ab13496 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

Would like to try a VEGFC antibody. High background in WB with ab83339 and ab13496.

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Answer

Thanks for your call today and for letting us know about the trouble with ab83339 and ab13496. Please keep me updated about any progress with these antibodies, and let me know if the protocol alterations we discussed are not helpful. Also, we have a few VEGFC antibodies that have been tested in WB with human samples, but ab9546 is our most frequently ordered and has been used in 6 publications- https://www.abcam.com/VEGFC-antibody-ab9546.html Please let me know if you would like to try this antibody or a different one and I can issue a discount code for you. I look forward to hearing from you. If you have any questions don't hesitate to ask! Have a great day.  

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Western blot abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain homogenate)
Loading amount
10 µg
Specification
Whole brain homogenate
Gel Running Conditions
Reduced Denaturing (Bis-tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Mr. Brian Hitt

Verified customer

Submitted May 07 2010

Immunohistochemistry - Free Floating abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Read More

Mr. Brian Hitt

Verified customer

Submitted Apr 30 2010

Question

When this antibody was tested in IHC, was it tested with formalin fixed paraffin embedded sections, frozen sections, or both?

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Answer

Thank you for your enquiry. Ab13496 has been tested for application in IHC-frozen sections and has not yet been tested in IHC-formalin fixed paraffin embedded sections. If you have any additional questions, please contact us again.

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