Overview

  • Product name
    Anti-Alpha B Crystallin antibody [1B6.1-3G4]
    See all Alpha B Crystallin primary antibodies
  • Description
    Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
  • Host species
    Mouse
  • Specificity
    There is no reactivity with Beta-crystallin, AlphaA-crystallin, Hsp25, Hsp27 or Hsp47 proteins.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC, IP, Flow Cyt, IHC-FrFl, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Cow Alpha B Crystallin.

  • Positive control
    • Recombinant Bovine alpha B Crystallin Protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab13496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FrFl 1/500.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    May contribute to the transparency and refractive index of the lens.
  • Tissue specificity
    Lens as well as other tissues.
  • Involvement in disease
    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similarities
    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Images

  • All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : alpha Crystallin

    Lane 3 : alpha A Crystallin

    Lane 4 : alpha B Crystallin
    Lane 5 : beta Crystallin

    Predicted band size: 21 kDa

  • Immunohistochemical analysis of human heart tissue using ab13496 at the concentration 10ug/ml.

  • Immunohistochemical analysis of murine skeletal muscle tissue, staining Alpha B Crystallin with ab13496. Sections were blocked for 1 hour in 1% BSA and 10% FCS. The primary antibody was conjugated to AlexaFluor®594 before diluting 1/20 and incubating overnight at 4°C.
  • Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab13496 staining Alpha B Crystallin in mouse brain tissue by Immunohistochemistry (free floating sections). Samples were incubated with the primary antibody at a 1/500 dilution for 16 hours at 4°C. A goat anti-mouse IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/10000 dilution.

    See Abreview

References

This product has been referenced in:
  • Kuipers HF  et al. Phosphorylation of aB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114:E1745-E1754 (2017). Read more (PubMed: 28196893) »
  • Wang X  et al. Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia. Mol Ther Nucleic Acids 7:417-428 (2017). Read more (PubMed: 28624217) »
See all 26 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Rat Tissue sections (brain, 20 micron-thick free-floating sections)
Specification
brain, 20 micron-thick free-floating sections
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Marie-Caroline Cotel

Verified customer

Submitted May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (muscle)
Total protein in input
20 µg
Specification
muscle
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Apr 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain homogenate)
Loading amount
10 µg
Specification
Whole brain homogenate
Gel Running Conditions
Reduced Denaturing (Bis-tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Brian Hitt

Verified customer

Submitted May 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections

Mr. Brian Hitt

Verified customer

Submitted Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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