Overview

  • Product name
    Anti-Alpha B Crystallin antibody [1B6.1-3G4]
    See all Alpha B Crystallin primary antibodies
  • Description
    Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC, IP, Flow Cyt, IHC-FrFl, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Cow Alpha B Crystallin.

  • Positive control
    • Recombinant Bovine alpha B Crystallin Protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab13496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FrFl Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May contribute to the transparency and refractive index of the lens.
  • Tissue specificity
    Lens as well as other tissues.
  • Involvement in disease
    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similarities
    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Images

  • All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : alpha Crystallin

    Lane 3 : alpha A Crystallin

    Lane 4 : alpha B Crystallin
    Lane 5 : beta Crystallin

    Predicted band size: 21 kDa



    This image was generated using the ascites version of the product.

  • Immunohistochemical analysis of human heart tissue using ab13496 at the concentration 10ug/ml.

    This image was generated using the ascites version of the product.

  • Immunohistochemical analysis of murine skeletal muscle tissue, staining Alpha B Crystallin with ab13496. Sections were blocked for 1 hour in 1% BSA and 10% FCS. The primary antibody was conjugated to AlexaFluor®594 before diluting 1/20 and incubating overnight at 4°C.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • This image was generated using the ascites version of the product.

  • ab13496 staining Alpha B Crystallin in mouse brain tissue by Immunohistochemistry (free floating sections). Samples were incubated with the primary antibody at a 1/500 dilution for 16 hours at 4°C. A goat anti-mouse IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/10000 dilution.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:
  • Borch JS  et al. Expanding the phenotype of filamin-C-related myofibrillar myopathy. Clin Neurol Neurosurg 176:30-33 (2019). Read more (PubMed: 30496909) »
  • Bolus DJ  et al. Recurrent heat shock impairs the proliferation and differentiation of C2C12 myoblasts. Cell Stress Chaperones 23:399-410 (2018). Read more (PubMed: 29063376) »
See all 30 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Rat Tissue sections (brain, 20 micron-thick free-floating sections)
Specification
brain, 20 micron-thick free-floating sections
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Marie-Caroline Cotel

Verified customer

Submitted May 28 2014

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (muscle)
Total protein in input
20 µg
Specification
muscle
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Apr 19 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain homogenate)
Loading amount
10 µg
Specification
Whole brain homogenate
Gel Running Conditions
Reduced Denaturing (Bis-tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Brian Hitt

Verified customer

Submitted May 07 2010

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections

Mr. Brian Hitt

Verified customer

Submitted Apr 30 2010

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