Anti-Alpha B Crystallin antibody (ab65940)
Key features and details
- Goat polyclonal to Alpha B Crystallin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-Alpha B Crystallin antibody
See all Alpha B Crystallin primary antibodies -
Description
Goat polyclonal to Alpha B Crystallin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Pig, Cynomolgus monkey, Orangutan
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Immunogen
Synthetic peptide:
RLEKDRFSVNLD
, from the internal region of human alpha B Crystallin -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab65940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 18-19 kDa (predicted molecular weight: 20 kDa). A 1 hour primary incubation is recommended for this product. Approx 22kDa band observed in Mouse and Rat Eye lysates (calculated MW of 20.1kDa according to Mouse NP_034094.1 and Rat NP_037067.1). |
Target
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Function
May contribute to the transparency and refractive index of the lens. -
Tissue specificity
Lens as well as other tissues. -
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 396089 Chicken
- Entrez Gene: 281719 Cow
- Entrez Gene: 102128339 Cynomolgus monkey
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100172850 Orangutan
- Entrez Gene: 100127443 Pig
- Entrez Gene: 100009295 Rabbit
- Entrez Gene: 25420 Rat
see all -
Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
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Western blot - Anti-Alpha B Crystallin antibody (ab65940)All lanes : Anti-Alpha B Crystallin antibody (ab65940) at 0.05 µg/ml
Lane 1 : Mouse eye lysate
Lane 2 : Rat eye lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 20 kDa
Datasheets and documents
References (0)
ab65940 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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