- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Alpha B Crystallin antibody
See all Alpha B Crystallin primary antibodies -
DescriptionGoat polyclonal to Alpha B Crystallin
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Host speciesGoat
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Pig, Cynomolgus monkey, Orangutan
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Immunogen
Synthetic peptide:
RLEKDRFSVNLD
, from the internal region of human alpha B Crystallin -
Positive control
- WB: Mouse and rat eye lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab65940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| WB |
Application notes
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18-19 kDa (predicted molecular weight: 20 kDa).
Peptide ELISA: antibody detection limit dilution 1:128,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1:128,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionMay contribute to the transparency and refractive index of the lens.
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Tissue specificityLens as well as other tissues.
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Involvement in diseaseDefects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
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Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
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Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
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Database links
- Entrez Gene: 396089 Chicken
- Entrez Gene: 281719 Cow
- Entrez Gene: 102128339 Cynomolgus monkey
- Entrez Gene: 1410 Human
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100172850 Orangutan
- Entrez Gene: 100127443 Pig
- Entrez Gene: 100009295 Rabbit
see all -
Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
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Western blot - Anti-Alpha B Crystallin antibody (ab65940)All lanes : Anti-Alpha B Crystallin antibody (ab65940) at 0.05 µg/ml
Lane 1 : Mouse eye lysate
Lane 2 : Rat eye lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 20 kDa
Protocols
References
ab65940 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"