Anti-Alpha B Crystallin antibody (ab65940)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Alpha B Crystallin antibody
See all Alpha B Crystallin primary antibodies -
Description
Goat polyclonal to Alpha B Crystallin -
Host species
Goat -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Pig, Cynomolgus monkey, Orangutan
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Immunogen
Synthetic peptide:
RLEKDRFSVNLD
, from the internal region of human alpha B Crystallin -
Positive control
- WB: Mouse and rat eye lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab65940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| WB |
Application notes
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18-19 kDa (predicted molecular weight: 20 kDa).
Peptide ELISA: antibody detection limit dilution 1:128,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1:128,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
May contribute to the transparency and refractive index of the lens. -
Tissue specificity
Lens as well as other tissues. -
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 396089 Chicken
- Entrez Gene: 281719 Cow
- Entrez Gene: 102128339 Cynomolgus monkey
- Entrez Gene: 1410 Human
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100172850 Orangutan
- Entrez Gene: 100127443 Pig
- Entrez Gene: 100009295 Rabbit
see all -
Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
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Western blot - Anti-Alpha B Crystallin antibody (ab65940)All lanes : Anti-Alpha B Crystallin antibody (ab65940) at 0.05 µg/ml
Lane 1 : Mouse eye lysate
Lane 2 : Rat eye lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 20 kDa
Protocols
Datasheets and documents
References
ab65940 has not yet been referenced specifically in any publications.
