Recombinant Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2752] to Alpha B Crystallin
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Alpha B Crystallin antibody [EPR2752]
See all Alpha B Crystallin primary antibodies -
Description
Rabbit monoclonal [EPR2752] to Alpha B Crystallin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human Alpha B Crystallin (C terminal). The exact sequence is proprietary.
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Positive control
- Fetal heart and rat eyeball lysates; human heart muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2752 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 20 kDa.
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IHC-P | (1) |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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IP |
1/250.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 20 kDa. |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250. |
IP
1/250. |
Target
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Function
May contribute to the transparency and refractive index of the lens. -
Tissue specificity
Lens as well as other tissues. -
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 1410 Human
- Entrez Gene: 25420 Rat
- Omim: 123590 Human
- SwissProt: P02511 Human
- SwissProt: P23928 Rat
- Unigene: 53454 Human
- Unigene: 98208 Rat
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Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
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Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Rat eyeball lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Alpha B Crystallin with purified ab76467 at 1/250. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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Immunohistochemical staining of paraffin embedded human brain with purified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Human fetal heart tissue lysate at 10 µg/ml
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunofluorescence staining of HeLa cells with purified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
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ab76467 (purified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemical staining of paraffin embedded human brain with unpurified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Immunohistochemical staining of alpha B Crystallin in paraffin embedded human heart muscle with ab76467 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Lane 1 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/5000 dilution
Lane 2 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/20000 dilution
Lane 1 : fetal heart lysate
Lane 2 : rat eyeball lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Immunofluorescence staining of HeLa cells with unpurified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
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ab76467 (unpurified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab76467 has been referenced in 5 publications.
- Dorgau B et al. Human Retinal Organoids Provide a Suitable Tool for Toxicological Investigations: A Comprehensive Validation Using Drugs and Compounds Affecting the Retina. Stem Cells Transl Med 11:159-177 (2022). PubMed: 35298655
- Zhu X et al. Characterization of ferroptosis signature to evaluate the predict prognosis and immunotherapy in glioblastoma. Aging (Albany NY) 13:17655-17672 (2021). PubMed: 34244461
- Muraleva NA et al. SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32731533
- Zeng L et al. Prognostic value of biomarkers EpCAM and aB-crystallin associated with lymphatic metastasis in breast cancer by iTRAQ analysis. BMC Cancer 19:831 (2019). PubMed: 31443698
- Bódi B et al. Titin isoforms are increasingly protected against oxidative modifications in developing rat cardiomyocytes. Free Radic Biol Med 113:224-235 (2017). PubMed: 28943453