Recombinant Anti-Alpha B Crystallin antibody [SP288] - BSA and Azide free (ab272007)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP288] to Alpha B Crystallin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Alpha B Crystallin antibody [SP288] - BSA and Azide free
See all Alpha B Crystallin primary antibodies -
Description
Rabbit monoclonal [SP288] to Alpha B Crystallin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human renal cell carcinoma, melanoma, kidney, cerebellum, spinal cord and heart tissues.
-
General notes
ab272007 is the carrier-free version of ab226839.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP288 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
Primary incubation for 10 minutes at room temperature.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Primary incubation for 10 minutes at room temperature.
|
Target
-
Function
May contribute to the transparency and refractive index of the lens. -
Tissue specificity
Lens as well as other tissues. -
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
-
Database links
- Entrez Gene: 281719 Cow
- Entrez Gene: 1410 Human
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100127443 Pig
- Entrez Gene: 25420 Rat
- Omim: 123590 Human
- SwissProt: P02510 Cow
- SwissProt: P02511 Human
see all -
Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for Alpha B Crystallin using ab226839 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226839). -
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Alpha B Crystallin using ab226839 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226839). -
Formalin-fixed, paraffin-embedded human spinal cord tissue stained for Alpha B Crystallin using ab226839 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226839).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab272007 has not yet been referenced specifically in any publications.