Overview

  • Product name
    Anti-Alpha B Crystallin (phospho S19) antibody
    See all Alpha B Crystallin primary antibodies
  • Description
    Rabbit polyclonal to Alpha B Crystallin (phospho S19)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Sheep, Rabbit, Hamster, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Alpha B Crystallin aa 14-25.
    Sequence: FFPFHSpPSRLFD

  • Positive control
    • Immunofluorescence staining of phospho-alpha-B crystallin (Ser19) in U373 MG cells results in cytoplasmic staining.

Properties

Applications

Our Abpromise guarantee covers the use of ab5597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.

Target

Images

  • All lanes : Anti-Alpha B Crystallin (phospho S19) antibody (ab5597) at 0.5 µg/ml

    Lane 1 : phospho-alpha-B crystallin (Ser19)
    Lane 2 : unphosphorylated alpha-B crystallin

    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    Western blot of recombinant bovine phospho-alpha-B crystallin (Ser19) using ab5597. Lane 1 is phospho-alpha-B crystallin (Ser19) and lane 2 is unphosphorylated alpha-B crystallin.

References

This product has been referenced in:
  • Lambrecht S  et al. Differential expression of alphaB-crystallin and evidence of its role as a mediator of matrix gene expression in osteoarthritis. Arthritis Rheum 60:179-88 (2009). WB ; Human . Read more (PubMed: 19116904) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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