Overview

  • Product name
  • Description
    Rabbit polyclonal to alpha COP I
  • Host species
    Rabbit
  • Specificity
    Detects coatomer-protein I alpha (COP I alpha).
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Rat, Human, Non human primates
    Predicted to work with: Mouse, Cow, Dog, Rice
  • Immunogen

    Synthetic peptide corresponding to Rat alpha COP I aa 1-19.
    Sequence:

    MLTKFETKSARVKGLSFHP


    (Peptide available as ab4931)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100, 140 kDa.Can be blocked with alpha COP I peptide (ab4931).

Target

  • Function
    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
    Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.
  • Tissue specificity
    Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.
  • Sequence similarities
    Contains 6 WD repeats.
  • Developmental stage
    Xenin is released into the circulation after a meal.
  • Cellular localization
    Secreted and Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha coat protein antibody
    • Alpha COP antibody
    • Alpha COPI antibody
    • Alpha-coat protein antibody
    • Alpha-COP antibody
    • AlphaCOP antibody
    • Coatomer protein complex subunit alpha antibody
    • Coatomer subunit alpha antibody
    • COP A antibody
    • COP I alpha antibody
    • copA antibody
    • COPA_HUMAN antibody
    • COPI alpha antibody
    • FLJ26320 antibody
    • HEP COP antibody
    • HEP-COP antibody
    • HEPCOP antibody
    • Proxenin antibody
    • Xenin antibody
    • Xenopsin-related peptide antibody
    see all

Images

  • ICC/IF of COP1 in Hela Cells

References

This product has been referenced in:
  • Sudo H  et al. Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model. Genomics 95:210-6 (2010). WB ; Human . Read more (PubMed: 20153416) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for the explanation, and for the image and protocol. The staining you are seeing with the alpha COP anditboy ab2913 does look incorrect, if all the staining is expected to be peri-nuclear, as is seen with the delta COP antibody. The small black and white image of HeLa cells on the ab2913 datasheet also shows what appears to be intra-nuclear staining, along with the ER/Golgi stain. This suggests that the antibody routinely cross-reacts with a nuclear protein that is not alpha COP (unless the apparent nuclear stain is actually COP on top of the nuclei). We have not received other complaints, but few people have tried ab2913. I suggest replacing the antibody with another, but the only other alpha COP antibody in our catalogue is ab104710, and this has only been tested for western blotting. It may work for ICC, but you will need to optimize the stain. If you are interested in trying this, I will send a vial free-of-charge, to replace ab2913. Click here (or use the following: https://www.abcam.com/index.html?datasheet=104710). If you prefer, I can send an antibody against a different target, for instance the beta COP antibody ab2899: Click here (or use the following: https://www.abcam.com/index.html?datasheet=2899). I can also issue a credit or refund, if you prefer. I look forward to your reply.

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Answer

Thank you for contacting us. I am sorry to read that you are having trouble getting a good result with this antibody. Can you please tell me what application you are using it for? I am assuming IHC or ICC but I will need a few more details. We always ask for sample and protocol information in these instances, in the hope that we can make a suggestion that improves the result. If the application and species are covered by our Abpromise guarantee, and the antibody was purchased within the past 6 months, we can send a replacement or refund if we we are unable to suggest a solution for the problem.

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Answer

Thank you for your email. Ab2913 was tested for application in Western blotting using PC-12 cell extract. The antibody detected a band at approximately 140 kDa representing COPI alpha. The antibody also detected a band at approximately 100 kDa, the identity of which is unknown. We currently do not have an image, but we do guarantee that the antibody will work in Western blotting and cross-react with human and rat alpha COP I. I would recommend using PC-12 cell extract as a positive control and we also sell the blocking peptide (ab4931) which can be used in control experiments. Please contact us again if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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