Key features and details
- Rabbit polyclonal to alpha COP I/COPA - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-alpha COP I/COPA antibody - C-terminal
See all alpha COP I/COPA primary antibodies
DescriptionRabbit polyclonal to alpha COP I/COPA - C-terminal
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
This product was previously labelled as alpha COP I
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192919 was affinity purified using an epitope specific to alpha COP I/COPA immobilized on solid support.
Our Abpromise guarantee covers the use of ab192919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 138 kDa.|
FunctionThe coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.
Tissue specificityUniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.
Sequence similaritiesContains 6 WD repeats.
Developmental stageXenin is released into the circulation after a meal.
Cellular localizationSecreted and Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
- Information by UniProt
- Alpha coat protein antibody
- Alpha COP antibody
- Alpha COPI antibody
All lanes : Anti-alpha COP I/COPA antibody - C-terminal (ab192919) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 138 kDa
Exposure time: 3 minutes
ab192919 has not yet been referenced specifically in any publications.