Overview

  • Product name

    Anti-alpha COP I/COPA antibody [EPR14273(B)]
    See all alpha COP I/COPA primary antibodies
  • Description

    Rabbit monoclonal [EPR14273(B)] to alpha COP I/COPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha COP I/COPA aa 1-100. The exact sequence is proprietary.
    Database link: P53621

  • Positive control

    • HeLa, Jurkat, MCF7 and HepG2 whole cell lysate (ab7900); Human Endometrium tissue.
  • General notes

     

     

     This product was previously labelled as alpha COP I

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14273(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 138 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
    Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.
  • Tissue specificity

    Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.
  • Sequence similarities

    Contains 6 WD repeats.
  • Developmental stage

    Xenin is released into the circulation after a meal.
  • Cellular localization

    Secreted and Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha coat protein antibody
    • Alpha COP antibody
    • Alpha COPI antibody
    • Alpha-coat protein antibody
    • Alpha-COP antibody
    • AlphaCOP antibody
    • Coatomer protein complex subunit alpha antibody
    • Coatomer subunit alpha antibody
    • COP A antibody
    • COP I alpha antibody
    • copA antibody
    • COPA_HUMAN antibody
    • COPI alpha antibody
    • FLJ26320 antibody
    • HEP COP antibody
    • HEP-COP antibody
    • HEPCOP antibody
    • Proxenin antibody
    • Xenin antibody
    • Xenopsin-related peptide antibody
    see all

Images

  • All lanes : Anti-alpha COP I/COPA antibody [EPR14273(B)] (ab181224) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 138 kDa

  • Immunohistochemical analysis of paraffin-embedded Human Endometrium tissue labeling alpha COP I/COPA with ab181224 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

ab181224 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181224.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up