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    alpha-defensin-1-2-3-antibody-ab99504.pdf

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Microbiology Protein Human Protein Defensin
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Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
  • Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)

Key features and details

  • Goat polyclonal to alpha Defensin 1 + 2 + 3
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-alpha Defensin 1 + 2 + 3 antibody
  • Description

    Goat polyclonal to alpha Defensin 1 + 2 + 3
  • Host species

    Goat
  • Specificity

    ab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-DESLAPKHPGSRKN

    , corresponding to internal sequence amino acids 50-63 of Human alpha Defensin 1 + 2 + 3 (NP_004075.1; NP_005208.1).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human spleen lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Protein
    • Human Protein
    • Defensin
    • Immunology
    • Secreted Molecules
    • Other secreted molecules

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

Our Abpromise guarantee covers the use of ab99504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa.

1 hour primary incubation is recommended for this product.

Target

  • Relevance

    Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 1667 Human
    • Omim: 125220 Human
    • SwissProt: P59665 Human
    • Alternative names

      • DEF1 antibody
      • DEF3 antibody
      • DEFA1 antibody
      • DEFA2 antibody
      • DEFA3 antibody
      • Defensin alpha 1 antibody
      • Defensin alpha 2 antibody
      • Defensin alpha 3 antibody
      • HNP1 antibody
      • HNP3 antibody
      • HP1 antibody
      • HP3 antibody
      • MRS antibody
      • Neutrophil defensin 1 antibody
      • Neutrophil defensin 2 antibody
      • Neutrophil defensin 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
      Immunohistochemical analysis of paraffin embedded Human Thymus staining alpha Defensin 1 + 2 + 3 with ab99504 (3.8µg/ml). Steamed antigen retrieval with citrate buffer pH 6.
    • Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
      Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
      Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 10 kDa


      Exposure time: 1 hour
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
      ab99504 (3.8µg/ml) staining of paraffin embedded Human Thymus shows staining of selected cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab99504? Please let us know so that we can cite the reference in this datasheet.

    ab99504 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    1-2 of 2 Q&A

    Question

    Je recherche un anticorps anti-defensin/cryptdin pour une utilisation en IHC-P sur du tissu de souris.

    J'ai repéré le Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) ou Anti-alpha Defensin NP5 antibody [8C8] (ab62757) qui reconnaissent l'humain.

    Avez vous des infos sur sa compatibilité avec le tissu murin?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 26 2013

    Answer



    Nous n’avons jamais testé ni ab99504 ni ab62757 chez la souris en IHC-P.

    J’ai fait une comparaison entre l’alpha Defensin 1 + 2 + 3 humaine et murin et il n’y qu’une homologie totale d’environ 30%. J’ai aussi fait une comparaison entre la protéine humaine et murin alpha Defensin NP5 et j’ai trouvé que la protéine totale avait 33% d’homologie. Cela suggère que malheureusement aucun anticorps anti-humain alpha Defensin fonctionnera sur des tissue murins.

    Read More

    Abcam Scientific Support

    Answered on Mar 26 2013

    Question

    searching for an anti NP 1 alpha defensin ELISA kit to test on human samples

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2011

    Answer

    Vielen Dank für Ihren Anruf und Ihr Interesse an unseren Produkten. Leider haben wir zur Zeit kein ELISA Kit in unserem Katalog gegen das NP-1/alpha defensin 1 protein. Wir haben drei Antikörper, die jedoch auch andere zyklische alpha Defensin erkennen können. Wir haben zur Zeit auch keinen Antikörper der nur das NP1 erkennen würde. ab90486: Dieser monokonale Antikörper aus der Maus erkennt das humane NP1, NP2 und NP3. Dieser Antikörper wurde auch in ELISA und Western blot getestet und ist darum auch von uns in diesen Applikationen garantiert. Click here (or use the following: https://www.abcam.com/alpha-Defensin-1-antibody-B539M-ab90486.html). ab99504: Dieser polyklonale Antikörper aus der Ziege wurde nur in Western blot getestet bislang, und erkennt auch die humanen NP1, NP2 und NP3. Falls Sie diesen Antikörper in ELISA testen möchten, könnten wir Ihnen ein Testangebot machen. Bitte lassen Sie mich wissen ob Sie dies Interessiert. Click here (or use the following: https://www.abcam.com/alpha-Defensin-1-2-3-antibody-ab99504.html). ab64763: Dieser monoklonale Antikörper aus der Maus erkennt auch NP1, NP2 und NP3 aus dem Menschen und wurde auch in ELISA und Western blot getestet. Click here (or use the following: https://www.abcam.com/alpha-Defensin-1-2-3-antibody-D1F4-ab64763.html). Bitte lassen Sie mich wissen falls Sie weitere Fragen haben oder ob das Testangebot Sie interessieren könnte. Ich wünsche Ihnen einen ganz schönen Nachmittag noch.

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2011

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