Key features and details
- Goat polyclonal to alpha Defensin 1 + 2 + 3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-alpha Defensin 1 + 2 + 3 antibody
DescriptionGoat polyclonal to alpha Defensin 1 + 2 + 3
Specificityab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Human spleen lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab99504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa.
1 hour primary incubation is recommended for this product.
RelevanceDefensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.
- DEF1 antibody
- DEF3 antibody
- DEFA1 antibody
Immunohistochemical analysis of paraffin embedded Human Thymus staining alpha Defensin 1 + 2 + 3 with ab99504 (3.8µg/ml). Steamed antigen retrieval with citrate buffer pH 6.
Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 10 kDa
Exposure time: 1 hour
ab99504 (3.8µg/ml) staining of paraffin embedded Human Thymus shows staining of selected cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab99504 has not yet been referenced specifically in any publications.