Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
Key features and details
- Goat polyclonal to alpha Defensin 1 + 2 + 3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Defensin 1 + 2 + 3 antibody -
Description
Goat polyclonal to alpha Defensin 1 + 2 + 3 -
Host species
Goat -
Specificity
ab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
C-DESLAPKHPGSRKN
, corresponding to internal sequence amino acids 50-63 of Human alpha Defensin 1 + 2 + 3 (NP_004075.1; NP_005208.1). -
Positive control
- Human spleen lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab99504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Relevance
Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 1667 Human
- Omim: 125220 Human
- SwissProt: P59665 Human
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Alternative names
- DEF1 antibody
- DEF3 antibody
- DEFA1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)Immunohistochemical analysis of paraffin embedded Human Thymus staining alpha Defensin 1 + 2 + 3 with ab99504 (3.8µg/ml). Steamed antigen retrieval with citrate buffer pH 6.
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Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 10 kDa
Exposure time: 1 hour -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)ab99504 (3.8µg/ml) staining of paraffin embedded Human Thymus shows staining of selected cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Protocols
Datasheets and documents
References (0)
ab99504 has not yet been referenced specifically in any publications.