• Product name

    Anti-alpha Defensin NP5 antibody [8C8]
  • Description

    Mouse monoclonal [8C8] to alpha Defensin NP5
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human alpha Defensin NP5.

  • Positive control

    • Human duodenum FFPE tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab62757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.


  • Function

    Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
  • Tissue specificity

    Paneth cells of the small intestine.
  • Sequence similarities

    Belongs to the alpha-defensin family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • alpha 5 antibody
    • Alpha defensin 5 antibody
    • DEF 5 antibody
    • DEF5 antibody
    • DEF5_HUMAN antibody
    • DEFA 5 antibody
    • DEFA5 antibody
    • Defensin 5 antibody
    • Defensin alpha 5 antibody
    • Defensin alpha 5 Paneth cell specific antibody
    • Defensin alpha 5 preproprotein antibody
    • Defensin antibody
    • Defensin-5 antibody
    • Defensin5 antibody
    • HD 5 antibody
    • HD5 antibody
    • HNP 5 antibody
    • HNP5 antibody
    • MGC129728 antibody
    • NP 5 antibody
    • NP5 antibody
    see all


  • IHC image of Defensin NP5 staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62757, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Stronati L  et al. Altered expression of innate immunity genes in different intestinal sites of children with ulcerative colitis. Dig Liver Dis 42:848-53 (2010). WB ; Human . Read more (PubMed: 20452301) »
  • Arijs I  et al. Mucosal gene expression of antimicrobial peptides in inflammatory bowel disease before and after first infliximab treatment. PLoS One 4:e7984 (2009). IHC-P ; Human . Read more (PubMed: 19956723) »
See all 4 Publications for this product

Customer reviews and Q&As


Nous n’avons jamais testé ni ab99504 ni ab62757 chez la souris en IHC-P.

J’ai fait une comparaison entre l’alpha Defensin 1 + 2 + 3 humaine et murin et il n’y qu’une homologie totale d’environ 30%. J’ai aussi fait une comparaison entre la protéine humaine et murin alpha Defensin NP5 et j’ai trouvé que la protéine totale avait 33% d’homologie. Cela suggère que malheureusement aucun anticorps anti-humain alpha Defensin fonctionnera sur des tissue murins.

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