Key features and details
- Mouse monoclonal [8C8] to alpha Defensin NP5
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-alpha Defensin NP5 antibody [8C8]
DescriptionMouse monoclonal [8C8] to alpha Defensin NP5
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human alpha Defensin NP5.
- Human duodenum FFPE tissue sections.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsAvoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab62757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
FunctionHas antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
Tissue specificityPaneth cells of the small intestine.
Sequence similaritiesBelongs to the alpha-defensin family.
- Information by UniProt
- alpha 5 antibody
- Alpha defensin 5 antibody
- DEF 5 antibody
IHC image of Defensin NP5 staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62757, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab62757 has been referenced in 5 publications.
- Haberman Y et al. Age-of-diagnosis dependent ileal immune intensification and reduced alpha-defensin in older versus younger pediatric Crohn Disease patients despite already established dysbiosis. Mucosal Immunol 12:491-502 (2019). PubMed: 30542108
- Stronati L et al. Altered expression of innate immunity genes in different intestinal sites of children with ulcerative colitis. Dig Liver Dis 42:848-53 (2010). WB ; Human . PubMed: 20452301
- Arijs I et al. Mucosal gene expression of antimicrobial peptides in inflammatory bowel disease before and after first infliximab treatment. PLoS One 4:e7984 (2009). IHC-P ; Human . PubMed: 19956723
- Wehkamp J et al. Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues. FEBS Lett 580:5344-50 (2006). PubMed: 16989824
- Wehkamp J et al. Reduced Paneth cell alpha-defensins in ileal Crohn's disease. Proc Natl Acad Sci U S A 102:18129-34 (2005). PubMed: 16330776