Overview

  • Product name
    Anti-alpha Elastin antibody
    See all alpha Elastin primary antibodies
  • Description
    Rabbit polyclonal to alpha Elastin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, ELISA, WB, IP, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Cow, Dog, Human
    Does not react with: Rat
  • Immunogen

    Full length native protein (insoluble alpha elastin purified from human aorta by hot sodium hydroxide).

Properties

Applications

Our Abpromise guarantee covers the use of ab21607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/100.
ELISA 1/1000 - 1/2000.
WB 1/200 - 1/500. Predicted molecular weight: 70 kDa.
IP 1/100 - 1/200.
IHC-FoFr 1/50 - 1/100. In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
IHC-P 1/50 - 1/100. In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.

Target

  • Function
    Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificity
    Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in disease
    Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1) [MIM:123700]. A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities
    Belongs to the elastin family.
  • Post-translational
    modifications
    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    • WBS antibody
    • WS antibody
    see all

References

This product has been referenced in:
See all 13 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (aorta)
Gel Running Conditions
Non-reduced Denaturing (8)
Loading amount
45 µg
Treatment
Ang II for 2 wks
Specification
aorta
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 19 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (human liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
human liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 12 2015

Answer

Thank you for your inquiry.

The lab said that they would think ab21607 & ab21610 would recognize the precursor of elastin, aka tropoelastin, but this hasn't been specifically tested.

I think I found an antibody that would work for you and that is ab21600, anti-tropoelastin. This works in mouse in ICC, IHC-Fr, IHC-P, ELISA, WB, andIP.

https://www.abcam.com/Tropoelastin-antibody-ab21600.html

I hope this information helps. Please contact us with any other questions.

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Question

LOT NUMBER gr9066-6 ORDER NUMBER 984557 DESCRIPTION OF THE PROBLEM Non-specific staining SAMPLE Species: mouse What’s cell line or tissue: skin PRIMARY ANTIBODY Species:rabbit polyclonal Reacts against: human mouse cow dog At what dilution(s) have you tested this antibody:1:50/1:500 Diluent buffer: DAKO antibody diluents buffer (ready to use) Incubation time: overnight Incubation temperature: +4℃ What washing steps were done (which buffer, number of washes): 1x PBS/3 times/5 mins DETECTION METHOD AEC ANTIBODY STORAGE CONDITIONS -20℃ FIXATION OF SAMPLE Type of sample (Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed ˙paraffin embedded sections, cells in culture, other:____) : PFA fixed paraffin embedded sections ANTIGEN RETRIEVAL Antigen retrieval method(including time, temperature etc.): 1X Tris-EDTA PH9 Time:15mins/10mins Temperature:99~100℃ PERMEABILIZATION STEP Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?No ˙Permeabilizing agent and concentration: BLOCKING CONDITIONS Blocking agent (eg BSA, serum…): superblocking buffer (pierce 37515) Concentration:ready to use Blocking time :1hr Blocking temperature: room temperature Endogenous peroxidases blocked? 3% H2O2 methanol room temperature 10 mins SECONDARY ANTIBODY Species:DAKO LSAB2 System Reacts against which species: mouse/rabbit/goat At what dilution(s) have you tested this antibody: Diluent buffer: Incubation time:biotinylated secondary antibody and streptavidin-HRP reagent 30mins/each What washing steps were done (which buffer, number of washes): 1x PBS/3 times/5 mins Fluorochrome or enzyme conjugate (eg: FITC, HRP, AP, biotin…etc): biotinylated secondary antibody and streptavidin-HRP reagent Signal amplification method (eg: ABC, LSAB, HRP polymer, TSE): LSAB HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? 1.Primary antibody diluent ratio 1:50/1:500 2.Antigen retrieval time 15mins/10mins

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Answer

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab21607. I would also appreciate if you can confirm some further details: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols. I would try including this step. A number of other researchers on IHC world have stained Elastin successfully be using proteinase K antigen retrieval at 37°C for 20 min. This is another step I would try but without the pretreatment detailed above. How was the antibody treated prior to storage at -20°C? Glycerol should be added to a final volume of 50% prior to storage. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

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Answer

Thank you for your reply. I can confirm that ab21607 alpha Elastin antibody has been tested and guaranteed for IHC-P and human and mouse samples. Therefore, this would be a suitable alternative antibody.  I would like to reassure you that we monitor feedback closely on a weekly basis and are not currently concerned about the general quality of ab77804 or of this batch.  We sell quite a lot of this antibody, and regrettably, I can suggest you have received a bad vial on this occasion.  I would be pleased to arrange an free of charge replacement, which would still be covered by our Abpromise guarantee. Alternatively, I understand you concerns and if you wish to receive an alternative free of charge vial of ab21607 I would be pleased to arrange this. Please let me know if you have any further questions, and I look forward to hearing from you with details of how you would like to proceed.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (endothelial cell)
Loading amount
30 µg
Specification
endothelial cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Guo-Ping Shi

Verified customer

Submitted Jul 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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