Overview

  • Product name

    Anti-alpha Elastin antibody
    See all alpha Elastin primary antibodies
  • Description

    Rabbit polyclonal to alpha Elastin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, ELISA, WB, IP, IHC-FoFr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rabbit, Cow, Dog, Human
    Does not react with: Rat
  • Immunogen

    Full length native protein (insoluble alpha elastin purified from human aorta by hot sodium hydroxide).

Properties

Applications

Our Abpromise guarantee covers the use of ab21607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/100.
ELISA 1/1000 - 1/2000.
WB 1/200 - 1/500. Predicted molecular weight: 70 kDa.
IP 1/100 - 1/200.
IHC-FoFr 1/50 - 1/100. In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
IHC-P 1/50 - 1/100. In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.

Target

  • Function

    Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificity

    Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in disease

    Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1) [MIM:123700]. A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities

    Belongs to the elastin family.
  • Post-translational
    modifications

    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links

  • Alternative names

    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    • WBS antibody
    • WS antibody
    see all

References

This product has been referenced in:

  • Ouyang A  et al. Chronic exercise training prevents coronary artery stiffening in aortic-banded miniswine: role of perivascular adipose-derived advanced glycation end products. J Appl Physiol (1985) 127:816-827 (2019). Read more (PubMed: 31295062) »
  • Wrenn SM  et al. Avian lungs: A novel scaffold for lung bioengineering. PLoS One 13:e0198956 (2018). Read more (PubMed: 29949597) »
See all 15 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Mouse Tissue lysate - whole (aorta)
Gel Running Conditions
Non-reduced Denaturing (8)
Loading amount
45 µg
Treatment
Ang II for 2 wks
Specification
aorta
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 19 2016

Application
Western blot
Sample
Human Tissue lysate - whole (human liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
human liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 12 2015

Application
Western blot
Sample
Mouse Cell lysate - other (endothelial cell)
Loading amount
30 µg
Specification
endothelial cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Guo-Ping Shi

Verified customer

Submitted Jul 13 2008

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