Western blot abreview for Anti-alpha Elastin antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (endothelial cell)
Loading amount
30 µg
Specification
endothelial cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: rabbit polyclonal antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: ~72 kDa

Additional data

Additional Notes
We used 1:200 dilution. Therefore, this product is expensive, but works great.

Dr. Guo-Ping Shi

Verified customer

Submitted Jul 13 2008

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