Western blot abreview for Anti-alpha Elastin antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (aorta)
Gel Running Conditions
Non-reduced Denaturing (8)
Loading amount
45 µg
Treatment
Ang II for 2 wks
Specification
aorta
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 210, 170, 130 kDa

Additional data

PubMed ID
25657308: View

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Submitted Dec 19 2016

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