Anti-GANAB antibody (ab96757)
Key features and details
- Rabbit polyclonal to GANAB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GANAB antibody
See all GANAB primary antibodies -
Description
Rabbit polyclonal to GANAB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human GANAB aa 97-261.
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and NIH-3T3 whole cell lysates; HeLa cells; SW480 xenograft; human breasr carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 107 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. -
Tissue specificity
Detected in placenta. -
Pathway
Glycan metabolism; N-glycan metabolism. -
Sequence similarities
Belongs to the glycosyl hydrolase 31 family. -
Cellular localization
Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 23193 Human
- Entrez Gene: 14376 Mouse
- Omim: 104160 Human
- SwissProt: Q14697 Human
- SwissProt: Q8BHN3 Mouse
- Unigene: 595071 Human
- Unigene: 3196 Mouse
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Alternative names
- Alpha glucosidase II alpha subunit antibody
- Alpha-glucosidase 2 antibody
- G2AN antibody
see all
Images
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Mehanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells staibed forGANAB using ab96757 at1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-Alpha tubulin antibody (red).
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All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : GANAB knockout HeLa cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG HRP
Predicted band size: 107 kDa -
Paraffin-embedded human breast carcinoma tissue stained for GANAB using ab96757 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Anti-GANAB antibody (ab96757) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 107 kDa
7.5% SDS PAGE -
All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : Molt-4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 107 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of GANAB in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.
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Immunofluorescence analysis of GANAB in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96757 has not yet been referenced specifically in any publications.