Product nameAnti-alpha Glucosidase II antibody [EPR12376]
See all alpha Glucosidase II primary antibodies
DescriptionRabbit monoclonal [EPR12376] to alpha Glucosidase II
Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human alpha Glucosidase II aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14697
- HeLa, Human placenta, A431 and HepG2 lysates. Paraffin-embedded Human gastric carcinoma tissue, paraffin-embedded Human placenta tissue, permeabilized Hela cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 107 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/50 - 1/100.|
FunctionCleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
Tissue specificityDetected in placenta.
PathwayGlycan metabolism; N-glycan metabolism.
Sequence similaritiesBelongs to the glycosyl hydrolase 31 family.
Cellular localizationEndoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Alpha glucosidase II alpha subunit antibody
- Alpha-glucosidase 2 antibody
- G2AN antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: alpha Glucosidase II knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab176349 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab176349 was shown to specifically react with alpha Glucosidase II when alpha Glucosidase II knockout samples were used. Wild-type and alpha Glucosidase II knockout samples were subjected to SDS-PAGE. ab176349 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Flow cytometric analysis of permeabilized Hela cells labeling Glucosidase II with ab176349 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent staining of HeLa cells labeling alpha Glucosidase II with ab176349 at 1/50 dilution (red). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling alpha Glucosidase II with ab176349 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Humangastric carcinoma tissue labeling alpha Glucosidase II with ab176349 at 1/50 dilution.
All lanes : Anti-alpha Glucosidase II antibody [EPR12376] (ab176349) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human placenta lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 107 kDa
ab176349 has not yet been referenced specifically in any publications.