Overview

  • Product name
    Anti-alpha Glucosidase II antibody [EPR12376]
    See all alpha Glucosidase II primary antibodies
  • Description
    Rabbit monoclonal [EPR12376] to alpha Glucosidase II
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human alpha Glucosidase II aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q14697

  • Positive control
    • HeLa, Human placenta, A431 and HepG2 lysates. Paraffin-embedded Human gastric carcinoma tissue, paraffin-embedded Human placenta tissue, permeabilized Hela cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
    • Tissue specificity
      Detected in placenta.
    • Pathway
      Glycan metabolism; N-glycan metabolism.
    • Sequence similarities
      Belongs to the glycosyl hydrolase 31 family.
    • Cellular localization
      Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha glucosidase II alpha subunit antibody
      • Alpha-glucosidase 2 antibody
      • G2AN antibody
      • GANAB antibody
      • GANAB_HUMAN antibody
      • Glu II antibody
      • Glucosidase alpha neutral AB antibody
      • Glucosidase II alpha antibody
      • Glucosidase II subunit alpha antibody
      • GluII antibody
      • KIAA0088 antibody
      • Neutral alpha glucosidase AB antibody
      • Neutral alpha glucosidase AB precursor antibody
      • Neutral alpha-glucosidase AB antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: alpha Glucosidase II knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab176349 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.


      ab176349 was shown to specifically react with alpha Glucosidase II when alpha Glucosidase II knockout samples were used. Wild-type and alpha Glucosidase II knockout samples were subjected to SDS-PAGE. ab176349 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow cytometric analysis of permeabilized Hela cells labeling Glucosidase II with ab176349 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Immunofluorescent staining of HeLa cells labeling alpha Glucosidase II with ab176349 at 1/50 dilution (red). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling alpha Glucosidase II with ab176349 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Humangastric carcinoma tissue labeling alpha Glucosidase II with ab176349 at 1/50 dilution.

    • All lanes : Anti-alpha Glucosidase II antibody [EPR12376] (ab176349) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human placenta lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 107 kDa

    References

    ab176349 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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