Key features and details
- Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal
- Suitable for: WB, ELISA, Neutralising
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Product nameAnti-alpha-hemolysin antibody [8B7] - N-terminal
DescriptionMouse monoclonal [8B7] to alpha-hemolysin - N-terminal
Specificityab190467 does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, ab190467 interacts with Protein A in S. aureus culture supernatant via the Fc region.
Tested applicationsSuitable for: WB, ELISA, Neutralisingmore details
Species reactivityReacts with: Staphylococcus aureus
Synthetic peptide corresponding to alpha-hemolysin (N terminal). Polypeptide sequence of Staphylococcus aureus alpha-hemolysin targeting the N terminus of the mature toxin.
Database link: P09616
- Alpha Hemolysin Toxin; culture supernatant of USA300 strain
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab190467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.|
|ELISA||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration.
ab190467 can neutralize the hemolytic activity of the toxin.
RelevanceAlpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
Cellular localizationSecreted. Note: Secreted as a monomer. After oligomerization and pore formation, the complex is translocated across the bilayer, probably via the Gly-rich domain of each strand.
- Alpha-HL antibody
- Alpha-toxin antibody
- hla antibody
- hly antibody
Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of ab190467 for neutralization of 0.3 μg/mL of alpha-hemolysin was determined to be 0.676 μg/mL.
All lanes : Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) at 1 µg/ml
Lane 1 : alpha-emolysin at 0.1 µg
Lane 2 : Culture supernatant of USA300
Lane 3 : Negative control USA300 delta Hla strain
Predicted band size: 36 kDa
ab190467 has been referenced in 2 publications.
- Johansson C et al. Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla. Virulence 10:502-510 (2019). PubMed: 31131704
- Wolfmeier H et al. Liposomal Therapy Attenuates Dermonecrosis Induced by Community-Associated Methicillin-Resistant Staphylococcus aureus by Targeting a-Type Phenol-Soluble Modulins and a-Hemolysin. EBioMedicine 33:211-217 (2018). PubMed: 29936135