Overview

  • Product name

    Anti-alpha-hemolysin antibody [8B7] - N-terminal
  • Description

    Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal
  • Host species

    Mouse
  • Specificity

    ab190467 does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, ab190467 interacts with Protein A in S. aureus culture supernatant via the Fc region.
  • Tested applications

    Suitable for: WB, Neutralisingmore details
  • Species reactivity

    Reacts with: Staphylococcus aureus
  • Immunogen

    Synthetic peptide corresponding to alpha-hemolysin (N terminal). Polypeptide sequence of Staphylococcus aureus alpha-hemolysin targeting the N terminus of the mature toxin.
    Database link: P09616

  • Positive control

    • Alpha Hemolysin Toxin; culture supernatant of USA300 strain

Properties

Applications

Our Abpromise guarantee covers the use of ab190467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
Neutralising Use at an assay dependent concentration.

ab190467 can neutralize the hemolytic activity of the toxin.

Target

  • Relevance

    Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
  • Cellular localization

    Secreted. Note: Secreted as a monomer. After oligomerization and pore formation, the complex is translocated across the bilayer, probably via the Gly-rich domain of each strand.
  • Database links

    • Alternative names

      • Alpha-HL antibody
      • Alpha-toxin antibody
      • hla antibody
      • hly antibody

    Images

    • Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of ab190467 for neutralization of 0.3 μg/mL of alpha-hemolysin was determined to be 0.676 μg/mL.

    • All lanes : Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) at 1 µg/ml

      Lane 1 : alpha-emolysin at 0.1 µg
      Lane 2 : Culture supernatant of USA300
      Lane 3 : Negative control USA300 delta Hla strain

      Predicted band size: 36 kDa

    References

    This product has been referenced in:

    • Wolfmeier H  et al. Liposomal Therapy Attenuates Dermonecrosis Induced by Community-Associated Methicillin-Resistant Staphylococcus aureus by Targeting a-Type Phenol-Soluble Modulins and a-Hemolysin. EBioMedicine 33:211-217 (2018). Read more (PubMed: 29936135) »
    See 1 Publication for this product

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