Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467)
Key features and details
- Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal
- Suitable for: WB, Neutralising
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Overview
-
Product name
Anti-alpha-hemolysin antibody [8B7] - N-terminal -
Description
Mouse monoclonal [8B7] to alpha-hemolysin - N-terminal -
Host species
Mouse -
Specificity
ab190467 does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, ab190467 interacts with Protein A in S. aureus culture supernatant via the Fc region. -
Tested applications
Suitable for: WB, Neutralisingmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Synthetic peptide corresponding to alpha-hemolysin (N terminal). Polypeptide sequence of Staphylococcus aureus alpha-hemolysin targeting the N terminus of the mature toxin.
Database link: P09616 -
Positive control
- Alpha Hemolysin Toxin; culture supernatant of USA300 strain
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8B7 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
|
|
Neutralising |
Use at an assay dependent concentration.
ab190467 can neutralize the hemolytic activity of the toxin. |
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. |
Neutralising
Use at an assay dependent concentration. ab190467 can neutralize the hemolytic activity of the toxin. |
Target
-
Relevance
Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity. -
Cellular localization
Secreted. Note: Secreted as a monomer. After oligomerization and pore formation, the complex is translocated across the bilayer, probably via the Gly-rich domain of each strand. -
Database links
- Entrez Gene: 1123836 Staphylococcus aureus
- Entrez Gene: 3920722 Staphylococcus aureus
- SwissProt: P09616 Staphylococcus aureus
- SwissProt: Q2G1X0 Staphylococcus aureus
-
Alternative names
- Alpha-HL antibody
- Alpha-toxin antibody
- hla antibody
- hly antibody
Images
-
Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of ab190467 for neutralization of 0.3 μg/mL of alpha-hemolysin was determined to be 0.676 μg/mL.
-
All lanes : Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) at 1 µg/ml
Lane 1 : alpha-emolysin at 0.1 µg
Lane 2 : Culture supernatant of USA300
Lane 3 : Negative control USA300 delta Hla strain
Predicted band size: 36 kDa
Datasheets and documents
-
Datasheet download
References (7)
ab190467 has been referenced in 7 publications.
- Saenz-de-Juano MD et al. Inflammatory Response of Primary Cultured Bovine Mammary Epithelial Cells to Staphylococcus aureus Extracellular Vesicles. Biology (Basel) 11:N/A (2022). PubMed: 35336789
- Ziesemer S et al. Staphylococcus aureus Alpha-Toxin in Deep Tracheal Aspirates-Preliminary Evidence for Its Presence in the Lungs of Sepsis Patients. Toxins (Basel) 14:N/A (2022). PubMed: 35878188
- Mayer K et al. Within-Host Adaptation of Staphylococcus aureus in a Bovine Mastitis Infection Is Associated with Increased Cytotoxicity. Int J Mol Sci 22:N/A (2021). PubMed: 34445550
- Chu AJ et al. Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus. Int J Mol Sci 21:N/A (2020). PubMed: 32796751
- Jordan PM et al. Staphylococcus aureus-Derived a-Hemolysin Evokes Generation of Specialized Pro-resolving Mediators Promoting Inflammation Resolution. Cell Rep 33:108247 (2020). PubMed: 33053344
- Johansson C et al. Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla. Virulence 10:502-510 (2019). PubMed: 31131704
- Wolfmeier H et al. Liposomal Therapy Attenuates Dermonecrosis Induced by Community-Associated Methicillin-Resistant Staphylococcus aureus by Targeting a-Type Phenol-Soluble Modulins and a-Hemolysin. EBioMedicine 33:211-217 (2018). PubMed: 29936135