• Product name

    Anti-alpha Internexin antibody [2E3]
    See all alpha Internexin primary antibodies
  • Description

    Mouse monoclonal [2E3] to alpha Internexin
  • Host species

  • Specificity

    specifically recognizes a-internexin.
  • Tested applications

    Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Cow, Human
    Predicted to work with: Mammals
  • Immunogen

    Recombinant full length protein corresponding to Rat alpha Internexin. (Purified from E. coli).

  • Epitope

    C-terminal non-helical extension of the protein.
  • Positive control

    • WB: Rat and mouse brain and spinal cord lysates; Cow spinal cord lysate. Flow Cyt: SH-SY5Y cells.



Our Abpromise guarantee covers the use of ab7654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 66 kDa.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ICC/IF 1/500.
IHC-P 1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.


  • Function

    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • Tissue specificity

    Found predominantly in adult CNS.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all


  • All lanes : Anti-alpha Internexin antibody [2E3] (ab7654) at 1/10000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat spinal cord lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse spinal cord lysate
    Lane 5 : Cow spinal cord lysate

    Predicted band size: 66 kDa

  • ab7654 staining alpha Internexin in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA, pH 9.0 (100°C for 20 minutes). Samples were incubated with primary antibody (1/2500) for 20 minutes at 25°C.

    See Abreview

  • Hippocampal neurons in tissue culture with neurofilament alpha internexin (red) (Ab7654) and GFAP (green). Photo courtesy of: Dr. Gerry Shaw University of Florida
  • All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)

    Lanes 1-2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
    Lanes 3-4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
    Lanes 5-6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
    Lanes 7-8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
    Lanes 9-10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

  • Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:

  • Rauch U  et al. Expression of intermediate filament proteins and neuronal markers in the human fetal gut. J Histochem Cytochem 54:39-46 (2006). Read more (PubMed: 16087706) »
  • Uchikado H  et al. Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005). Read more (PubMed: 15883343) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 100C, 20 min
Human Tissue sections (cerebral cortex)
cerebral cortex

Abcam user community

Verified customer

Submitted Jul 01 2014


Thank you for contacting us about this issue. I am tempted to say you are detecting internexin +/- phosphorylation but the bands appear to be too far apart to fit that explanation. Do you have an image with the molecular weight markers, assuming you included them in the gel?

What are you using for a secondary antibody? If anti-mouse IgG, have you tried staining a blot of these samples with just the secondary antibody, to check the possibility that one of the bands is the heavy chain of endogenous mouse IgG, assuming your samples are reduced and denatured?

Read More
Western blot
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 06 2012

Immunohistochemistry (Frozen sections)
Apteronotus leptorhynchus Tissue sections (Brain)
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Apr 12 2012


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit an Abreview for Apteronotus leptorhynchus and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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