Product nameAnti-alpha Internexin antibody [2E3]
See all alpha Internexin primary antibodies
DescriptionMouse monoclonal [2E3] to alpha Internexin
Specificityspecifically recognizes a-internexin.
Tested applicationsSuitable for: IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Rat, Cow, Human
Predicted to work with: Mammals
Recombinant full length protein corresponding to Rat alpha Internexin. (Purified from E. coli).
EpitopeC-terminal non-helical extension of the protein.
- WB: Rat and mouse brain and spinal cord lysates; Cow spinal cord lysate. Flow Cyt: SH-SY5Y cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab7654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/100. Predicted molecular weight: 66 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
FunctionClass-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Tissue specificityFound predominantly in adult CNS.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageExpressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 66 kDa neurofilament protein antibody
- AINX_HUMAN antibody
- Alpha Inx antibody
All lanes : Anti-alpha Internexin antibody [2E3] (ab7654) at 1/10000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spinal cord lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse spinal cord lysate
Lane 5 : Cow spinal cord lysate
Predicted band size: 66 kDa
ab7654 staining alpha Internexin in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA, pH 9.0 (100°C for 20 minutes). Samples were incubated with primary antibody (1/2500) for 20 minutes at 25°C.
Hippocampal neurons in tissue culture with neurofilament alpha internexin (red) (Ab7654) and GFAP (green). Photo courtesy of: Dr. Gerry Shaw University of Florida
All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)
Lanes 1-2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
Lanes 3-4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
Lanes 5-6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
Lanes 7-8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
Lanes 9-10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury
Predicted band size: 66 kDa
Observed band size: 66 kDa
Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Rauch U et al. Expression of intermediate filament proteins and neuronal markers in the human fetal gut. J Histochem Cytochem 54:39-46 (2006). Read more (PubMed: 16087706) »
- Uchikado H et al. Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005). Read more (PubMed: 15883343) »