For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant full length protein corresponding to Rat alpha Internexin. (Purified from E. coli).
Our Abpromise guarantee covers the use of ab7654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/100. Predicted molecular weight: 66 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
ab7654 staining alpha Internexin in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA, pH 9.0 (100°C for 20 minutes). Samples were incubated with primary antibody (1/2500) for 20 minutes at 25°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"