Key features and details
- Rabbit polyclonal to alpha Internexin
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-alpha Internexin antibody
See all alpha Internexin primary antibodies
DescriptionRabbit polyclonal to alpha Internexin
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human alpha Internexin aa 300-350. The exact sequence is proprietary.
Database link: Q16352
- IP: HEK-293T whole cell lysate.
ab226368 has not performed satisfactorily when used for WB of alpha Internexin in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of alpha Internexin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226368 was affinity purified using an epitope specific to alpha Internexin immobilized on solid support.
Our Abpromise guarantee covers the use of ab226368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionClass-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Tissue specificityFound predominantly in adult CNS.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageExpressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 66 kDa neurofilament protein antibody
- AINX_HUMAN antibody
- Alpha Inx antibody
alpha Internexin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226368 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226368 at 0.4 µg/ml.
Lane 1: ab226368 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226368 has not yet been referenced specifically in any publications.