Overview

  • Product name

    Anti-alpha Internexin antibody
    See all alpha Internexin primary antibodies
  • Description

    Rabbit polyclonal to alpha Internexin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human alpha Internexin (internal sequence). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: Q16352

  • Positive control

    • WB: U-87 MG, SK-N-SH, SK-N-AS and SK-SY5Y whole cell lysate; mouse and rat brain lysate. IP: U-87 MG whole cell lysate. ICC/IF: Rat E18 primary cortical neuron, DIV 8 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/800.
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.

Target

  • Function

    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • Tissue specificity

    Found predominantly in adult CNS.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • Post-translational
    modifications

    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

Images

  • All lanes : Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
    Lane 3 : SK-N-AS whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    10% SDS-PAGE

  • Rat E18 primary cortical neuron DIV 8 cells stained for alpha Internexin (red) using ab227614 at 1/250 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Blue: Fluoroshield with DAPI.

  • Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution + SK-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    10% SDS-PAGE

  • Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    10% SDS-PAGE

  • Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution + Rat brain tissue lysate at 50 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    10% SDS-PAGE

  • alpha Internexin was immunoprecipitated from U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with 5 μg ab227614. Western blot was performed from the immunoprecipitate using ab227614. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: IgG instead of ab227614 in U-87 MG whole cell lysate

    Lane 2: ab227614 IP in U-87 MG whole cell lysate.

References

ab227614 has not yet been referenced specifically in any publications.

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