Overview

  • Product name
    Anti-alpha Internexin antibody
    See all alpha Internexin primary antibodies
  • Description
    Chicken polyclonal to alpha Internexin
  • Host species
    Chicken
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human
  • Immunogen

    Recombinant full length protein expressed in E. coli.

  • Positive control
    • ICC/IF: Rat cerebellum. WB: Rat brain and spinal cord; Mouse brain and spinal cord; Cow spinal cord; Pig spinal cord.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.065% Sodium azide
  • Concentration information loading...
  • Purification notes
    This antibody was purified from egg yolk by chloroform delipidation followed by PEG precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 66-68 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/1000.

Target

  • Function
    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • Tissue specificity
    Found predominantly in adult CNS.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Developmental stage
    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • Post-translational
    modifications
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

Images

  • Immunofluorescent analysis of a rat cerebellum section stained with ab24523 (Red) at 1/5000 dilution in immunohistochemical analysis. counterstained with antibody against myelin basic protein (Green). Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. ab24523 stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.

  • All lanes : Anti-alpha Internexin antibody (ab24523) at 1/10000 dilution

    Lane 1 : Protein standard (red)
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spinal cord tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse spinal cord tissue lysate
    Lane 6 : Cow spinal cord tissue lysate
    Lane 7 : Pig spinal cord tissue lysate

    Predicted band size: 55 kDa



    ab24523 reveals the α-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.

References

ab24523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab24523.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up