The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000. Detects a band of approximately 66-68 kDa (predicted molecular weight: 55 kDa).
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Found predominantly in adult CNS.
Belongs to the intermediate filament family.
Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
O-glycosylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunofluorescent analysis of a rat cerebellum section stained with ab24523 (Red) at 1/5000 dilution in immunohistochemical analysis. counterstained with antibody against myelin basic protein (Green). Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. ab24523 stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.
Western blot - Anti-alpha Internexin antibody (ab24523)
All lanes : Anti-alpha Internexin antibody (ab24523) at 1/10000 dilution
Lane 1 : Protein standard (red) Lane 2 : Rat brain tissue lysate Lane 3 : Rat spinal cord tissue lysate Lane 4 : Mouse brain tissue lysate Lane 5 : Mouse spinal cord tissue lysate Lane 6 : Cow spinal cord tissue lysate Lane 7 : Pig spinal cord tissue lysate
Predicted band size: 55 kDa
ab24523 reveals the α-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.