Overview

  • Product name

    Anti-alpha Internexin antibody
    See all alpha Internexin primary antibodies
  • Description

    Rabbit polyclonal to alpha Internexin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human alpha Internexin (C terminal).

  • Positive control

    • Fetal brain lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Flow Cyt
  • Application notes
    Flow Cyt: 1/20.
    IP: 1/100.
    WB: 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 55 kDa).

    Is unsuitable for IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
    • Tissue specificity

      Found predominantly in adult CNS.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Developmental stage

      Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
    • Post-translational
      modifications

      O-glycosylated.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links

    • Alternative names

      • 66 kDa neurofilament protein antibody
      • AINX_HUMAN antibody
      • Alpha Inx antibody
      • Alpha-internexin antibody
      • Alpha-Inx antibody
      • INA antibody
      • Internexin neuronal intermediate filament protein alpha antibody
      • MGC12702 antibody
      • NEF 5 antibody
      • NEF5 antibody
      • Neurofilament 5 (66kD) antibody
      • Neurofilament 5 antibody
      • Neurofilament 66 antibody
      • Neurofilament 66 tax binding protein antibody
      • Neurofilament-66 antibody
      • NF 66 antibody
      • NF-66 antibody
      • NF66 antibody
      • TXBP 1 antibody
      • TXBP1 antibody
      see all

    Images

    • Anti-alpha Internexin antibody (ab52867) at 1/50000 dilution + Fetal brain lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 66 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Che Z  et al. Targeting CAND1 promotes caspase-8/RIP1-dependent apoptosis in liver cancer cells. Am J Transl Res 10:1357-1372 (2018). Read more (PubMed: 29887951) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab52867.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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