Overview

  • Product name
    Anti-alpha Internexin antibody [EP676Y]
    See all alpha Internexin primary antibodies
  • Description
    Rabbit monoclonal [EP676Y] to alpha Internexin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, WB, Flow Cyt, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Does not react with: Apteronotus leptorhynchus
  • Immunogen

    Synthetic peptide within Human alpha Internexin aa 400-500. The exact sequence is proprietary.

  • Positive control
    • SHSY5Y cell lysate, human brain
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP676Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 66 kDa).
Flow Cyt 1/25.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.

Target

  • Function
    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • Tissue specificity
    Found predominantly in adult CNS.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Developmental stage
    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • Post-translational
    modifications
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

Images

  • Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution + SHSY5Y cell lysate at 10 µg

    Predicted band size: 66 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • ab40758 staining alpha Internexin in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with 0.01% Triton X-100, blocked with 5% serum for 2 hours at 4°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/250) at 4°C for 10 hours. An undiluted Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.
  • ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1:250.

References

This product has been referenced in:
  • Iqbal J  et al. Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention. J Neurosci Res 96:1798-1815 (2018). Read more (PubMed: 30054946) »
  • Ayyadevara S  et al. Proteins that mediate protein aggregation and cytotoxicity distinguish Alzheimer's hippocampus from normal controls. Aging Cell 15:924-39 (2016). IHC-P ; Human . Read more (PubMed: 27448508) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Human Tissue (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Dec 29 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (Mixed Glial cells)
Specification
Mixed Glial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 21 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.01% Triton X100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 17 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Rat Cell (Glial Cells)
Specification
Glial Cells
Permeabilization
Yes - Saponin
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 13 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 06 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Apr 12 2012

Answer


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit an Abreview for Apteronotus leptorhynchus and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Vlady Pavlova

Verified customer

Submitted Dec 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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