Recombinant Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free
See all alpha Internexin primary antibodies -
Description
Rabbit monoclonal [EPR1529] to alpha Internexin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human alpha Internexin (C terminal). The exact sequence is proprietary.
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General notes
Ab247623 is the carrier-free version of ab108302. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247623 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR1529 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
Target
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Function
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. -
Tissue specificity
Found predominantly in adult CNS. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation. -
Post-translational
modificationsO-glycosylated.
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 9118 Human
- Entrez Gene: 226180 Mouse
- Entrez Gene: 24503 Rat
- Omim: 605338 Human
- SwissProt: Q16352 Human
- SwissProt: P46660 Mouse
- SwissProt: P23565 Rat
- Unigene: 500916 Human
see all -
Alternative names
- 66 kDa neurofilament protein antibody
- AINX_HUMAN antibody
- Alpha Inx antibody
see all
Datasheets and documents
References
ab247623 has not yet been referenced specifically in any publications.
