Product nameAlpha Ketoglutarate (alpha KG) Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids
Assay typeEnzyme activity (quantitative)
Sensitivity= 0.01 nmol/well
Range0.01 nmol/well - 10 nmol/well
Assay time0h 30m
Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.
In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe to a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product.
The alpha-Ketoglutarate assay kit can be used to measure alpha-ketoglutarate in the range of 0.01 to 10 nmoles.
Alpha Ketoglutarate assay protocol summary:
- add reaction mix to standard and sample wells
- incubate for 30 min
- analyze with a microplate reader
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests a-KG Assay Buffer WM 1 x 25ml a-KG Converting Enzyme (lyophilized) Purple 1 vial a-KG Development Enzyme Mix (lyophilized) Green 1 vial a-KG OxiRed Probe (in DMSO) Red 1 x 200µl a-KG Standard (10 µmol, lyophilized) Yellow 1 vial
RelevanceAlpha ketoglutarate (Alpha-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing Alpha-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. Alpha-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism.
- Alpha KG
This product has been referenced in:
- Wang J et al. miR-9-5p inhibits pancreatic cancer cell proliferation, invasion and glutamine metabolism by targeting GOT1. Biochem Biophys Res Commun 509:241-248 (2019). Read more (PubMed: 30591220) »
- Hong R et al. Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation. Mol Oncol 13:959-977 (2019). Read more (PubMed: 30714292) »