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we do not have a 535/587 nm filter for our plate reader, however we do have a 595 nm filter. Do you think that this filter will work with this kit? Do you know the whole spectrum of emission for the secondary antibody in this kit?
Asked on Oct 26 2011
This is a fluorescent assay and you will have to measure at two wavelengths: 535 nm and 587 nm. If you only have the 595 nm and not the 535 nm you will not be able to use the fluorescent measurements. However, this assay can also be measured colorimetrically (lmax = 570nm). So if your 595 nm filter has a bandwidth option of +/- 25, you can measure at the required 570 nm and be able to capture 100% of the signal.
Answered on Oct 26 2011