Question (51414) | Alpha Ketoglutarate (alpha KG) Assay Kit (ab83431)

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Question

The protocol says, "Tissue (20 mg) or cells (2 x 10^6) are rapidly homogenized with 100 μl of ice cold PBS or other buffer (pH 6.5 - 8)." I know this means using a Dounce homogenizer, but I'm using T cells and I'm worried this won't lyse effectively. Can I use RIPA buffer?

Answer

Thank you for your inquiry.

Yes, buffers with pH range 6.5 – 8 can be used. Just make sure that the RIPA buffer is within the recommendedpH.
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