Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 100 µU
Product nameα-Ketoglutarate Dehydrogenase Activity Assay Kit (Colorimetric)
Sample typeTissue, Adherent cells, Suspension cells
Sensitivity< 100 µU
Abcam’s α-Ketoglutarate Dehydrogenase Activity Assay kit (Colorimetric) (ab185440) provides a quick and easy way for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
α-Ketoglutarate Dehydrogenase (α-KGDH) (EC 220.127.116.11) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH converts α-ketoglutarate into succinylCoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that α-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests KGDH Assay Buffer 1 x 25ml KGDH Developer 1 vial KGDH Positive Control 1 x 50µl KGDH Substrate 1 vial NADH Standard ( lyophilized) Yellow 1 vial
FunctionThe 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Sequence similaritiesBelongs to the alpha-ketoglutarate dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 2 oxoglutarate dehydrogenase
- 2 oxoglutarate dehydrogenase complex component E1
- 2 oxoglutarate dehydrogenase mitochondrial
This is example data only.
α-Ketoglutarate Dehydrogenase activity in rat heart (75 µg) and liver lysates (100 µg). Assays were performed following the kit protocol. This is example data only.
α-Ketoglutarate Dehydrogenase specific activity was calculated in rat heart lysate (75 µg), yeast mitochondria prepared from S. Cerevisiae(10 µg) and in rat liver lysate (100 µg). Assays were performed following the kit protocol. This is example data only.
ab185440 has been referenced in 1 publication.
- Zeitlow K et al. The biological foundation of the genetic association of TOMM40 with late-onset Alzheimer's disease. Biochim Biophys Acta 1863:2973-2986 (2017). PubMed: 28768149