Overview

  • Product name
    α-Ketoglutarate Dehydrogenase Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Semi-quantitative
  • Sensitivity
    < 100 µU
  • Product overview

    Abcam’s α-Ketoglutarate Dehydrogenase Activity Assay kit (Colorimetric) (ab185440) provides a quick and easy way for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.

  • Notes

    α-Ketoglutarate Dehydrogenase (α-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH converts α-ketoglutarate into succinylCoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that α-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit.

  • Platform
    Microplate reader

Properties

Images

  • This is example data only.

  • α-Ketoglutarate Dehydrogenase activity in rat heart (75 µg) and liver lysates (100 µg). Assays were performed following the kit protocol. This is example data only.

  • α-Ketoglutarate Dehydrogenase specific activity was calculated in rat heart lysate (75 µg), yeast mitochondria prepared from S. Cerevisiae(10 µg) and in rat liver lysate (100 µg). Assays were performed following the kit protocol. This is example data only.

Protocols

References

This product has been referenced in:
  • Zeitlow K  et al. The biological foundation of the genetic association of TOMM40 with late-onset Alzheimer's disease. Biochim Biophys Acta 1863:2973-2986 (2017). Read more (PubMed: 28768149) »
See 1 Publication for this product

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