Product namealpha-L-Fucosidase Assay Kit
Sample typeSerum, Plasma, Cell Lysate, Tissue Lysate
Range1 mU/ml - 100 mU/ml
alpha-L-Fucosidase Assay Kit (ab272525) is a non-radioactive, colorimetric assay based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L for a 20 minute reaction.
Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments. Can be readily automated to measure thousands of samples per day.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 100 tests Standard (12.5 mM Nitrophenol) 1 x 1ml Stop Reagent 1 x 12ml Substrate Buffer 1 x 10ml
Cellular localizationFUCA1: Lysosome. FUCA2: Secreted.
Our Abpromise guarantee covers the use of ab272525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272525 has not yet been referenced specifically in any publications.