Anti-alpha Lactalbumin antibody (ab112972)

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Overview

  • Product name

    Anti-alpha Lactalbumin antibody
    See all alpha Lactalbumin primary antibodies

  • Description

    Goat polyclonal to alpha Lactalbumin

  • Host species

    Goat

  • Tested applications

    Suitable for: IHC-P, WB, ELISA, ICCmore details

  • Species reactivity

    Reacts with: Cow

  • Immunogen

    whole protein Bovine alpha-lactalbumin

Properties

Applications

Our Abpromise guarantee covers the use of ab112972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/30000. Predicted molecular weight: 16 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
ELISA 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1000-1/30000 dilution.
ICC 1/200 - 1/2000.

Target

  • Function

    Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.

  • Tissue specificity

    Mammary gland specific. Secreted in milk.

  • Sequence similarities

    Belongs to the glycosyl hydrolase 22 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • a-LACTA antibody
      • alfaLA antibody
      • Alpha-lactalbumin antibody
      • Lactalbumin alpha antibody
      • Lactose synthase B protein antibody
      • LALBA antibody
      • LALBA_HUMAN antibody
      • Lysozyme-like protein 7 antibody
      • LYZL7 antibody
      • MGC138521 antibody
      • MGC138523 antibody
      see all

    References

    ab112972 has not yet been referenced specifically in any publications.

    Publishing research using ab112972? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Dear Sir/Madam The customer will set ELISA application to detect the above 4 protein from human breast milk. 1. Beta-lactoglobulin : AB8226, AB8227 2. Alpha-lactalbumin : AB20212,AB112972, AB112973 3. Casein : AB47972 4. Lactoferrin : AB108882 Please kindly recommend technical or protocol and ab items No. suitable.

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    Answer

    Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ortholog, and to my knowledge, in most experimental settings the bovine protein is detected in human breast milk (e.g. PMID8163788). If this is of interest for the customer, I am happy to let you know that we have two antibodies against the bovine beta-lactoglobulin in our catalog (ab112893, ab112894). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112893). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112894). 2) Alpha-lactalbumin: Again, we have antibodies against the bovine protein, but also one antibody against the human protein which are guaranteed to work in ELISA (ab112972, ab112973, ab35288). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112972). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112973). Click here (or use the following: https://www.abcam.com/index.html?datasheet=35288). 3) Casein: We only have one antibody against the human Casein, suitable for ELISA (ab91167). Click here (or use the following: https://www.abcam.com/index.html?datasheet=91167). 4) Lactoferrin: We have a number of antibodies available against the human lactoferrin some of which are not only guaranteed to work in a conventional ELISA, but also in Sandwich ELISA (sELISA), for example ab10110 or ab15811. Click here (or use the following: https://www.abcam.com/index.html?datasheet=10110). Click here (or use the following: https://www.abcam.com/index.html?datasheet=15811). Please find attached our ELISA protocols. If the customer is interested in matched antibody pairs for Sandwich ELISA, or antibodies against these targets from other species than human, I would be pleased to assist in finding the most suitable ones. Please be reassured that all antibodies are covered by our Abpromise: We are happy to offer technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. All tested species and applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If the customer decides to go ahead and test these products in an untested species or application, please contact our Scientific Support team prior to the purchase as they might be eligible for our testing discount program. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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