Overview

  • Product name
    Anti-alpha Lactalbumin antibody [F20.16]
    See all alpha Lactalbumin primary antibodies
  • Description
    Mouse monoclonal [F20.16] to alpha Lactalbumin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Alpha Lactalbumin

  • General notes


    This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Purified IgM
  • Purification notes
    This antibody was purified using affinity chromatography. The sample was eluted using a commercially bought Neutral Elution Buffer and exchanged into PBS using a G25 column.
  • Primary antibody notes
    This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.
  • Clonality
    Monoclonal
  • Clone number
    F20.16
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgM
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa.
IHC-Fr Use at an assay dependent concentration.

Use withn acetone fixed tissues.

ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
  • Tissue specificity
    Mammary gland specific. Secreted in milk.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 22 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • a-LACTA antibody
    • alfaLA antibody
    • Alpha-lactalbumin antibody
    • Lactalbumin alpha antibody
    • Lactose synthase B protein antibody
    • LALBA antibody
    • LALBA_HUMAN antibody
    • Lysozyme-like protein 7 antibody
    • LYZL7 antibody
    • MGC138521 antibody
    • MGC138523 antibody
    see all

Images

  • ICC/IF image of ab20212 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Burchell J  et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). Read more (PubMed: 3905581) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

The histology was carried out on frozen sections (10ug) of lactating mammary glands.

It seems that lactating tissue is the most appropriate positive control, as normal and cancerous tissue does not appear to express alpha lactalbumin according to the human protein atlas file below:

http://www.proteinatlas.org/ENSG00000167531/tissue/staining+overview

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Answer

Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ortholog, and to my knowledge, in most experimental settings the bovine protein is detected in human breast milk (e.g. PMID8163788). If this is of interest for the customer, I am happy to let you know that we have two antibodies against the bovine beta-lactoglobulin in our catalog (ab112893, ab112894). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112893). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112894). 2) Alpha-lactalbumin: Again, we have antibodies against the bovine protein, but also one antibody against the human protein which are guaranteed to work in ELISA (ab112972, ab112973, ab35288). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112972). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112973). Click here (or use the following: https://www.abcam.com/index.html?datasheet=35288). 3) Casein: We only have one antibody against the human Casein, suitable for ELISA (ab91167). Click here (or use the following: https://www.abcam.com/index.html?datasheet=91167). 4) Lactoferrin: We have a number of antibodies available against the human lactoferrin some of which are not only guaranteed to work in a conventional ELISA, but also in Sandwich ELISA (sELISA), for example ab10110 or ab15811. Click here (or use the following: https://www.abcam.com/index.html?datasheet=10110). Click here (or use the following: https://www.abcam.com/index.html?datasheet=15811). Please find attached our ELISA protocols. If the customer is interested in matched antibody pairs for Sandwich ELISA, or antibodies against these targets from other species than human, I would be pleased to assist in finding the most suitable ones. Please be reassured that all antibodies are covered by our Abpromise: We are happy to offer technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. All tested species and applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If the customer decides to go ahead and test these products in an untested species or application, please contact our Scientific Support team prior to the purchase as they might be eligible for our testing discount program. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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