Product namealpha-Mannosidase Assay Kit
Sample typeCell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Lysate
Range1 mU/ml - 250 mU/ml
alpha-Mannosidase Assay Kit (ab272519) is a nonradioactive, colorimetric alpha-Mannosidase assay is based on the cleavage of 4-nitrophenol from a synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Fast and sensitive: Linear detection range (10 μL sample): 1 to 250 U/L for a 10 minute reaction.
Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 100 tests Standard (12.5 mM Nitrophenol) 1 x 1ml Stop Reagent 1 x 12ml Substrate Buffer 1 x 10ml
Our Abpromise guarantee covers the use of ab272519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272519 has not yet been referenced specifically in any publications.