Overview

  • Product name

  • Description

    Rabbit polyclonal to alpha MSH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic Human MSH alpha conjugated to Bovine thyroglobulin by a Glutaraldehyde linker (P01189).

  • Positive control

    • Rat pituitary gland tissue.
  • General notes

    For long-term storage, store lyophilized antibody until ready to use at -15º C or lower. After reconstitution, use immediately or refrigerate at 2º-8º C up to 2 days. For long-term storage, appropriately aliquot antibody to avoid repeated freeze/thaw cycles and freeze at -15° C or lower

Properties

Applications

Our Abpromise guarantee covers the use of ab123811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/4000 - 1/6000. in PBS/0.3% Triton X-100 using Bn/Av-HRP technique. Use 4% paraformaldehyde for Fixation and post-fixation.

Target

  • Relevance

    Melanocyte-stimulating hormone belongs to a group called the melanocortins. This group includes ACTH, alpha-MSH, beta-MSH and gamma-MSH; these peptides are all cleavage products of a large precursor peptide called pro-opiomelanocortin (POMC). Alpha-MSH is the most important melanocortin for pigmentation. It increases the pigmentation of skin by increasing melanin production in melanocytes.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Alpha MSH antibody
    • Melanotropin alpha antibody
    • POMC antibody
    • proopiomelanocortin antibody

Images

  • ab123811, at 1/4000, staining MSH alpha in rat pituitary gland tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Gao Y  et al. Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase. Mol Metab 7:155-160 (2018). IHC-P ; Mouse . Read more (PubMed: 29174000) »
  • Pravdivyi I  et al. Progressive postnatal decline in leptin sensitivity of arcuate hypothalamic neurons in the Magel2-null mouse model of Prader-Willi syndrome. Hum Mol Genet 24:4276-83 (2015). ICC/IF ; Mouse . Read more (PubMed: 25926624) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
Yes - 0,3% Triton X-100 in PBS
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 01 2014

Answer

We have not tested if this product will also detect POMC.

If you would like to know more about POMC and alpha MSH detection, please see the following publication link (Fig 2).

http://www.sciencedirect.com/science/article/pii/S0006899303028063

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Chicken brain cryosections)
Specification
Chicken brain cryosections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-0.1% Triton X-100
Blocking step
4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 08 2012

Answer

The customer is right and I was wrong. Sorry for the confusion.

DISCOUNT CODE: *******
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and testab123811 in Chicken. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forChicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.

The source of this antibody informed me that the immunogen is in the N-terminal region of the Human MSH and is unlikely to cross-react with Chicken. See attachment for more details about the homology between sequences.

I would not recommend this antibody or any other anti-MSH from our catalog for tests in Chicken .

I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. http://www.biocompare.com

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

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