Key features and details
- Rabbit polyclonal to alpha Sarcoglycan
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-alpha Sarcoglycan antibody
See all alpha Sarcoglycan primary antibodies
DescriptionRabbit polyclonal to alpha Sarcoglycan
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Hamster
- IHC-P: Human heart muscle tissue. WB: alpha Sarcoglycan overexpression HEK-293T cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Tissue specificityMost strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
Involvement in diseaseDefects in SGCA are the cause of limb-girdle muscular dystrophy type 2D (LGMD2D) [MIM:608099]; also known as Duchenne-like muscular dystrophy autosomal recessive type 2 or severe childhood autosomal recessive muscular dystrophy (SCARMD). LGMD2D is an autosomal recessive degenerative myopathy characterized by progressive muscle wasting from early childhood with loss of independent ambulation by teenage years. Muscle biopsy shows necrosis, decreased immunostaining for alpha sarcoglycan, and adhalin deficiency. The phenotype is less severe than LGMD2C.
Sequence similaritiesBelongs to the sarcoglycan alpha/epsilon family.
Cellular localizationCell membrane > sarcolemma. Cytoplasm > cytoskeleton.
- Information by UniProt
- 50 DAG antibody
- 50 kDa dystrophin associated glycoprotein antibody
- 50 kDa dystrophin-associated glycoprotein antibody
Formalin-fixed, paraffin-embedded human heart muscle tissue stained for alpha Sarcoglycan with ab244300 at a 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-alpha Sarcoglycan antibody (ab244300) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : alpha Sarcoglycan overexpression HEK-293T cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244300 has not yet been referenced specifically in any publications.