Overview

  • Product name
    Anti-alpha Sarcoglycan antibody [EPR14773]
    See all alpha Sarcoglycan primary antibodies
  • Description
    Rabbit monoclonal [EPR14773] to alpha Sarcoglycan
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human alpha Sarcoglycan aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16586

  • Positive control
    • Human skeletal muscle, mouse heart and rat heart lysates; Human skeletal muscle tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14773
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2000.

Perform heat mediated antigen retrieval with EDTA buffer pH 9.0 before commencing with IHC staining protocol.

WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
IP 1/30.

Target

  • Function
    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • Tissue specificity
    Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
  • Involvement in disease
    Defects in SGCA are the cause of limb-girdle muscular dystrophy type 2D (LGMD2D) [MIM:608099]; also known as Duchenne-like muscular dystrophy autosomal recessive type 2 or severe childhood autosomal recessive muscular dystrophy (SCARMD). LGMD2D is an autosomal recessive degenerative myopathy characterized by progressive muscle wasting from early childhood with loss of independent ambulation by teenage years. Muscle biopsy shows necrosis, decreased immunostaining for alpha sarcoglycan, and adhalin deficiency. The phenotype is less severe than LGMD2C.
  • Sequence similarities
    Belongs to the sarcoglycan alpha/epsilon family.
  • Cellular localization
    Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 50 DAG antibody
    • 50 kDa dystrophin associated glycoprotein antibody
    • 50 kDa dystrophin-associated glycoprotein antibody
    • 50DAG antibody
    • 50kD DAG antibody
    • 59kDa antibody
    • A2 antibody
    • adhalin antibody
    • ADL antibody
    • Alpha SG antibody
    • Alpha-sarcoglycan antibody
    • Alpha-SG antibody
    • Asg antibody
    • DAG2 antibody
    • DMDA2 antibody
    • Dystroglycan 2 antibody
    • Dystroglycan-2 antibody
    • LGMD2D antibody
    • sarcoglycan, alpha (dystrophin-associated glycoprotein) antibody
    • SCARMD1 antibody
    • Sgca antibody
    • SGCA_HUMAN antibody
    see all

Images

  • Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Immunhistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab189254 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunoprecipitation analysis of human skeletal muscle tissue lysate labeling alpha Sarcoglycan using ab189254 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human skeletal muscle tissue lysate.

References

This product has been referenced in:
  • Gerli MFM  et al. Combined Notch and PDGF Signaling Enhances Migration and Expression of Stem Cell Markers while Inducing Perivascular Cell Features in Muscle Satellite Cells. Stem Cell Reports 12:461-473 (2019). Read more (PubMed: 30745033) »
  • Egorova TV  et al. CRISPR/Cas9-generated mouse model of Duchenne muscular dystrophy recapitulating a newly identified large 430 kb deletion in the human DMD gene. Dis Model Mech 12:N/A (2019). Read more (PubMed: 31028078) »
See all 3 Publications for this product

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