Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control
See all Alpha Skeletal Muscle Actin primary antibodies -
Description
Mouse monoclonal [337CT 30.10.1] to Alpha Skeletal Muscle Actin - Loading Control -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken, Cow, Pig, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Alpha Skeletal Muscle Actin aa 1 to the C-terminus.
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Positive control
- This antibody gave a positive signal in HeLa and TE 671 whole cell lysates, and in the following tissue lysates: Human Heart (data not shown); Mouse Heart; Rat Heart; Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
337CT 30.10.1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab88226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block using 3% milk. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 281592 Cow
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 100154254 Pig
- Entrez Gene: 100009506 Rabbit
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
see all -
Alternative names
- a actin antibody
- ACTA antibody
- ACTA1 antibody
see all
Images
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Western blot - Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)All lanes : Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226) at 5 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Skeletal Muscle (Rat) Tissue Lysate
Lane 6 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds
Blocked using 3% milk.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hilse KE et al. The Expression of Uncoupling Protein 3 Coincides With the Fatty Acid Oxidation Type of Metabolism in Adult Murine Heart. Front Physiol 9:747 (2018). Read more (PubMed: 29988383) »