Key features and details
- Mouse monoclonal [337CT 30.10.1] to Alpha Skeletal Muscle Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control
See all Alpha Skeletal Muscle Actin primary antibodies
DescriptionMouse monoclonal [337CT 30.10.1] to Alpha Skeletal Muscle Actin - Loading Control
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken, Cow, Pig, Orangutan
Synthetic peptide corresponding to Human Alpha Skeletal Muscle Actin aa 1 to the C-terminus.
- This antibody gave a positive signal in HeLa and TE 671 whole cell lysates, and in the following tissue lysates: Human Heart (data not shown); Mouse Heart; Rat Heart; Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Clone number337CT 30.10.1
Light chain typekappa
Our Abpromise guarantee covers the use of ab88226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block using 3% milk.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- ACTA antibody
- ACTA1 antibody
All lanes : Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226) at 5 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Skeletal Muscle (Rat) Tissue Lysate
Lane 6 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds
Blocked using 3% milk.
ab88226 has been referenced in 2 publications.
- Moschinger M et al. Age-related sex differences in the expression of important disease-linked mitochondrial proteins in mice. Biol Sex Differ 10:56 (2019). PubMed: 31806023
- Hilse KE et al. The Expression of Uncoupling Protein 3 Coincides With the Fatty Acid Oxidation Type of Metabolism in Adult Murine Heart. Front Physiol 9:747 (2018). PubMed: 29988383