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    alpha-skeletal-muscle-actin-antibody-337ct-30101-loading-control-ab88226.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)

  • Datasheet
  • SDS
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Western blot - Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)

    Key features and details

    • Mouse monoclonal [337CT 30.10.1] to Alpha Skeletal Muscle Actin - Loading Control
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG1

    You may also be interested in

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    Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Protein
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    Recombinant Human Alpha Skeletal Muscle Actin protein (ab157838)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control
      See all Alpha Skeletal Muscle Actin primary antibodies
    • Description

      Mouse monoclonal [337CT 30.10.1] to Alpha Skeletal Muscle Actin - Loading Control
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Rabbit, Chicken, Cow, Pig, Orangutan
    • Immunogen

      Synthetic peptide corresponding to Human Alpha Skeletal Muscle Actin aa 1 to the C-terminus.

    • Positive control

      • This antibody gave a positive signal in HeLa and TE 671 whole cell lysates, and in the following tissue lysates: Human Heart (data not shown); Mouse Heart; Rat Heart; Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle.
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      337CT 30.10.1
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin
      • Cardiovascular
      • Heart
      • Contractility
      • Contractile Proteins
      • Actins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Alpha Skeletal Muscle Actin protein (ab157838)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab88226 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block using 3% milk.
    Notes
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block using 3% milk.

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similarities

      Belongs to the actin family.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P68133 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 281592 Cow
      • Entrez Gene: 58 Human
      • Entrez Gene: 11459 Mouse
      • Entrez Gene: 100154254 Pig
      • Entrez Gene: 100009506 Rabbit
      • Entrez Gene: 29437 Rat
      • Omim: 102610 Human
      • SwissProt: P68139 Chicken
      • SwissProt: P68138 Cow
      • SwissProt: P68133 Human
      • SwissProt: P68134 Mouse
      • SwissProt: P68137 Pig
      • SwissProt: P68135 Rabbit
      • SwissProt: P68136 Rat
      • Unigene: 1288 Human
      • Unigene: 214950 Mouse
      • Unigene: 82732 Rat
      see all
    • Alternative names

      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin, alpha skeletal muscle antibody
      • ACTS_HUMAN antibody
      • Alpha actin 1 antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • MPFD antibody
      • Skeletal muscle alpha actin 1 antibody
      see all

    Images

    • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)
      Western blot - Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)
      All lanes : Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226) at 5 µg/ml

      Lane 1 : Heart (Mouse) Tissue Lysate
      Lane 2 : Heart (Rat) Tissue Lysate
      Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
      Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
      Lane 5 : Skeletal Muscle (Rat) Tissue Lysate
      Lane 6 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
      Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 30 seconds


      Blocked using 3% milk.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab88226? Please let us know so that we can cite the reference in this datasheet.

    ab88226 has been referenced in 2 publications.

    • Moschinger M  et al. Age-related sex differences in the expression of important disease-linked mitochondrial proteins in mice. Biol Sex Differ 10:56 (2019). PubMed: 31806023
    • Hilse KE  et al. The Expression of Uncoupling Protein 3 Coincides With the Fatty Acid Oxidation Type of Metabolism in Adult Murine Heart. Front Physiol 9:747 (2018). PubMed: 29988383

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse embryo section)
    Specification
    mouse embryo section
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
    Blocking step
    NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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