Overview

  • Product name

    Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control
    See all Alpha Skeletal Muscle Actin primary antibodies
  • Description

    Mouse monoclonal [337CT 30.10.1] to Alpha Skeletal Muscle Actin - Loading Control
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Alpha Skeletal Muscle Actin aa 1 to the C-terminus.

  • Positive control

    • This antibody gave a positive signal in HeLa and TE 671 whole cell lysates, and in the following tissue lysates: Human Heart (data not shown); Mouse Heart; Rat Heart; Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab88226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block using 3% milk.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin alpha cardiac muscle antibody
    • Actin, alpha skeletal muscle antibody
    • ACTS_HUMAN antibody
    • Alpha actin 1 antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • Cardiac muscle alpha actin 1 antibody
    • MPFD antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • All lanes : Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226) at 5 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 5 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 6 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 30 seconds


    Blocked using 3% milk.

References

This product has been referenced in:

  • Hilse KE  et al. The Expression of Uncoupling Protein 3 Coincides With the Fatty Acid Oxidation Type of Metabolism in Adult Murine Heart. Front Physiol 9:747 (2018). Read more (PubMed: 29988383) »
See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse embryo section)
Specification
mouse embryo section
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

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Verified customer

Submitted May 02 2013

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