Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
![Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.com/ps/products/28/ab28052/Images/ab28052-415198-anti-alpha-skeletal-muscle-actin-antibody-immunocytochemistry-hela-human.jpg "Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)")
Key features and details
- Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgM
Overview
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Product name
Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]
See all Alpha Skeletal Muscle Actin primary antibodies -
Description
Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin -
Host species
Mouse -
Specificity
In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin). -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Purified striated muscle actin from rabbit.
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Positive control
- WB: Whole extract of Rat heart ICC: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.097% Sodium azide
Constituent: 0.79% Tris HCl -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
Alpha Sr-1 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
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| ICC |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. |
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ICC
Use at an assay dependent concentration. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 58 Human
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68133 Human
- SwissProt: P68136 Rat
- Unigene: 1288 Human
- Unigene: 82732 Rat
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Alternative names
- a actin antibody
- ACTA antibody
- ACTA1 antibody
see all
Images
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Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)Immunocytochemistry analysis of HeLa cells labeling Alpha Skeletal Muscle Actin with ab28052 at 1/1000. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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![Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.com/ps/products/28/ab28052/Images/ab28052-415199-anti-alpha-skeletal-muscle-actin-antibody-western-blot-heart-rat.jpg)
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)Lane 1 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/500 dilution
Lane 2 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/1000 dilution
Lane 3 : Mouse Monoclonal Anti-Actin (a-Sarcomeric) antibody at 1/500 dilution
All lanes : Whole extract of Rat heart
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 42 kDachemiluminescent substrate.
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![Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.com/ps/products/28/ab28052/Images/ab28052-38957-anti-alpha-skeletal-muscle-actin-antibody-alpha-sr-1-immunofluorescence.jpg)
Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.
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![Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.com/ps/products/28/ab28052/Images/ab28052-38956-anti-alpha-skeletal-muscle-actin-antibody-alpha-sr-1-western-blot.jpg)
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution
All lanes : 40 µg rat neonatal cardiac myocytes (whole cell lysate)
Secondary
All lanes : HRP polymer conjugated donkey anti-mouse IgG at 1/1000
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 second
This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (38)
ab28052 has been referenced in 38 publications.
- Yeo M & Kim G Micro/nano-hierarchical scaffold fabricated using a cell electrospinning/3D printing process for co-culturing myoblasts and HUVECs to induce myoblast alignment and differentiation. Acta Biomater 107:102-114 (2020). PubMed: 32142759
- Chen H et al. TGF-ß1/IL-11/MEK/ERK signaling mediates senescence-associated pulmonary fibrosis in a stress-induced premature senescence model of Bmi-1 deficiency. Exp Mol Med 52:130-151 (2020). PubMed: 31959867
- Wu T et al. OxLDL enhances choroidal neovascularization lesion through inducing vascular endothelium to mesenchymal transition process and angiogenic factor expression. Cell Signal 70:109571 (2020). PubMed: 32087305
- Paavola J et al. Vezf1 regulates cardiac structure and contractile function. EBioMedicine 51:102608 (2020). PubMed: 31911272
- Yang H et al. Legumain is a predictor of all-cause mortality and potential therapeutic target in acute myocardial infarction. Cell Death Dis 11:1014 (2020). PubMed: 33243972