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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (33)

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Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
  • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

Key features and details

  • Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
  • Suitable for: WB, ICC
  • Reacts with: Rat, Human
  • Isotype: IgM

You may also be interested in

Protein
Product image
Recombinant Human Alpha Skeletal Muscle Actin protein (ab157838)

View more associated products

Overview

  • Product name

    Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]
    See all Alpha Skeletal Muscle Actin primary antibodies
  • Description

    Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
  • Host species

    Mouse
  • Specificity

    In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Purified striated muscle actin from rabbit.

  • Positive control

    • WB: Whole extract of Rat heart ICC: HeLa cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.097% Sodium azide
    Constituent: 0.79% Tris HCl
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    Alpha Sr-1
  • Isotype

    IgM
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)
  • Positive Controls

    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Recombinant Protein

    • Recombinant Human Alpha Skeletal Muscle Actin protein (ab157838)
  • Related Products

    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
ICC
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
ICC
Use at an assay dependent concentration.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58 Human
    • Entrez Gene: 29437 Rat
    • Omim: 102610 Human
    • SwissProt: P68133 Human
    • SwissProt: P68136 Rat
    • Unigene: 1288 Human
    • Unigene: 82732 Rat
    • Alternative names

      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin, alpha skeletal muscle antibody
      • ACTS_HUMAN antibody
      • Alpha actin 1 antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • MPFD antibody
      • Skeletal muscle alpha actin 1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Immunocytochemistry analysis of HeLa cells labeling Alpha Skeletal Muscle Actin with ab28052 at 1/1000. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
    • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Lane 1 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/500 dilution
      Lane 2 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/1000 dilution
      Lane 3 : Mouse Monoclonal Anti-Actin (a-Sarcomeric) antibody at 1/500 dilution

      All lanes : Whole extract of Rat heart

      Secondary
      All lanes : Goat Anti-Mouse IgG-Peroxidase

      Predicted band size: 42 kDa



      chemiluminescent substrate.

    • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.

      ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

      This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

      See Abreview

    • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.
      All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution

      All lanes : 40 µg rat neonatal cardiac myocytes (whole cell lysate)

      Secondary
      All lanes : HRP polymer conjugated donkey anti-mouse IgG at 1/1000

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 1 second


      This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

      See Abreview

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (33)

    Publishing research using ab28052? Please let us know so that we can cite the reference in this datasheet.

    ab28052 has been referenced in 33 publications.

    • Yeo M & Kim G Micro/nano-hierarchical scaffold fabricated using a cell electrospinning/3D printing process for co-culturing myoblasts and HUVECs to induce myoblast alignment and differentiation. Acta Biomater 107:102-114 (2020). PubMed: 32142759
    • Chen H  et al. TGF-ß1/IL-11/MEK/ERK signaling mediates senescence-associated pulmonary fibrosis in a stress-induced premature senescence model of Bmi-1 deficiency. Exp Mol Med 52:130-151 (2020). PubMed: 31959867
    • Wu T  et al. OxLDL enhances choroidal neovascularization lesion through inducing vascular endothelium to mesenchymal transition process and angiogenic factor expression. Cell Signal 70:109571 (2020). PubMed: 32087305
    • Zheng Z  et al. CDKN2B upregulation prevents teratoma formation in multipotent fibromodulin-reprogrammed cells. J Clin Invest 129:3236-3251 (2019). PubMed: 31305260
    • Zhao Y  et al. LncRNA NR_003923 promotes cell proliferation, migration, fibrosis, and autophagy via the miR-760/miR-215-3p/IL22RA1 axis in human Tenon's capsule fibroblasts. Cell Death Dis 10:594 (2019). PubMed: 31391457
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin [Alpha Sr-1] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Rabbit Tissue sections (Heart)
    Specification
    Heart
    Permeabilization
    No
    Fixative
    Formalin 10%
    Read More

    Dr. Nawazish Naqvi

    Verified customer

    Submitted Jul 14 2014

    Question

    Hi We use a lot of your NBR1 antibody listed below. I was wondering if there was a possibility of ordering a larger size? perhaps 100ug or 200ug instead of 50ug? If not, perhaps a quote if we order 3 lots in 1 go? BW  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I can recommend to contact our customer service department with a completed  list of which antibodies and the quantity required for each product. They will be happy to then provide you with a quote. Please email: orders@abcam.com Thank you for your interest in our products. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart)
    Specification
    Heart
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 01 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

    Below Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Branchiostoma belcheri Tissue sections (whole-mount)
    Specification
    whole-mount
    Fixative
    Chilled 100% methanol or 4% paraformaldehyde/MOPS pH 7.5 overnight at 4°C, then stored in 75% Ethanol at -20°C
    Antigen retrieval step
    None
    Permeabilization
    Yes - 5% triton-X 100 / PBS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    金也 安井

    Verified customer

    Submitted May 10 2010

    Western blot abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Neonatal Cardiac Myocytes)
    Loading amount
    40 µg
    Specification
    Neonatal Cardiac Myocytes
    Treatment
    phenylephrine for 24 hours
    Gel Running Conditions
    Reduced Denaturing (10% polyacrylamide)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Richard Patten

    Verified customer

    Submitted Jun 02 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Neonatal Cardiac Myocytes)
    Specification
    Neonatal Cardiac Myocytes
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% NP40
    Blocking step
    2%BSA, 2% FBS in PBS with 0.1% NP40 as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Dr. Richard Patten

    Verified customer

    Submitted Jun 02 2008

    Question

    I would like to ask you for help. We need a specific antibody only labeling cardiomyocytes. If possible, the antibody should be labeled already. I have already bought the TroponinI antibody (ab97711) of Abcam which is working quite well. If you could offer another one as desribed, I would be glad. Thank you in advance for your help! Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Aug 31 2011

    Answer

    Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052) - N-Cadherin: N Cadherin antibody (ab12221) - alpha actinin; alpha Actinin antibody [0.T.02] (ab18061) - bFGF: FGF basic antibody (ab16828) I would recommend typing the target name in the search box provided on the Abcam homepage and then selecting the antibodies as per your requirements e.g. monoclonal, polyclonal, rabbit or mouse etc. The full list of Cardiomyocytes markers can be found by clicking the below link; http://www.antibodybeyond.com/reviews/cell-markers/cardiomyocyte-marker.htm Please click on the following link to explore the Abcam Cardiovascular page https://www.abcam.com/index.html?pageconfig=resource&rid=10694 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Aug 31 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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