Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

Reviews (5)Q&A (2)References (26)

Overview

  • Product name

    Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]
    See all Alpha Skeletal Muscle Actin primary antibodies

  • Description

    Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin

  • Host species

    Mouse

  • Specificity

    In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Human

  • Immunogen

    Purified striated muscle actin from rabbit.

Properties

Applications

Our Abpromise guarantee covers the use of ab28052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.

  • Sequence similarities

    Belongs to the actin family.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin alpha cardiac muscle antibody
    • Actin, alpha skeletal muscle antibody
    • ACTS_HUMAN antibody
    • Alpha actin 1 antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • Cardiac muscle alpha actin 1 antibody
    • MPFD antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Nawazish Naqvi

    ab28052 staining Alpha Skeletal Muscle Actin in rabbit heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 24 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Immunocytochemistry/ Immunofluorescence - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

    ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution

    All lanes : 40 µg rat neonatal cardiac myocytes (whole cell lysate)

    Secondary
    All lanes : HRP polymer conjugated donkey anti-mouse IgG at 1/1000

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 second


    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

References

This product has been referenced in:

  • Zhao S  et al. High-pressure carbon dioxide pneumoperitoneum induces oxidative stress and mitochondria-associated apoptotic pathway in rabbit kidneys with severe hydronephrosis. Int J Mol Med 43:305-315 (2019). Read more (PubMed: 30431064) »
  • Zhao Y  et al. LncRNA NR_003923 promotes cell proliferation, migration, fibrosis, and autophagy via the miR-760/miR-215-3p/IL22RA1 axis in human Tenon's capsule fibroblasts. Cell Death Dis 10:594 (2019). Read more (PubMed: 31391457) »
See all 26 Publications for this product

Publishing research using ab28052? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin [Alpha Sr-1] antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Rabbit Tissue sections (Heart)
Specification
Heart
Permeabilization
No
Fixative
Formalin 10%
Read More

Dr. Nawazish Naqvi

Verified customer

Submitted Jul 14 2014

Question

Hi We use a lot of your NBR1 antibody listed below. I was wondering if there was a possibility of ordering a larger size? perhaps 100ug or 200ug instead of 50ug? If not, perhaps a quote if we order 3 lots in 1 go? BW  

Read More
Answer

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I can recommend to contact our customer service department with a completed  list of which antibodies and the quantity required for each product. They will be happy to then provide you with a quote. Please email: orders@abcam.com Thank you for your interest in our products. If you have any further questions, please do not hesitate to contact us.

Read More

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Sep 01 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Below Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Branchiostoma belcheri Tissue sections (whole-mount)
Specification
whole-mount
Fixative
Chilled 100% methanol or 4% paraformaldehyde/MOPS pH 7.5 overnight at 4°C, then stored in 75% Ethanol at -20°C
Antigen retrieval step
None
Permeabilization
Yes - 5% triton-X 100 / PBS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

金也 安井

Verified customer

Submitted May 10 2010

Western blot abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Neonatal Cardiac Myocytes)
Loading amount
40 µg
Specification
Neonatal Cardiac Myocytes
Treatment
phenylephrine for 24 hours
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Richard Patten

Verified customer

Submitted Jun 02 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Neonatal Cardiac Myocytes)
Specification
Neonatal Cardiac Myocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% NP40
Blocking step
2%BSA, 2% FBS in PBS with 0.1% NP40 as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Read More

Dr. Richard Patten

Verified customer

Submitted Jun 02 2008

Question

I would like to ask you for help. We need a specific antibody only labeling cardiomyocytes. If possible, the antibody should be labeled already. I have already bought the TroponinI antibody (ab97711) of Abcam which is working quite well. If you could offer another one as desribed, I would be glad. Thank you in advance for your help! Kind regards

Read More
Answer

Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052) - N-Cadherin: N Cadherin antibody (ab12221) - alpha actinin; alpha Actinin antibody [0.T.02] (ab18061) - bFGF: FGF basic antibody (ab16828) I would recommend typing the target name in the search box provided on the Abcam homepage and then selecting the antibodies as per your requirements e.g. monoclonal, polyclonal, rabbit or mouse etc. The full list of Cardiomyocytes markers can be found by clicking the below link; http://www.antibodybeyond.com/reviews/cell-markers/cardiomyocyte-marker.htm Please click on the following link to explore the Abcam Cardiovascular page https://www.abcam.com/index.html?pageconfig=resource&rid=10694 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com