Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
- Datasheet
- References (18)
- Protocols
Overview
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Product nameAnti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]
See all Alpha Skeletal Muscle Actin primary antibodies -
DescriptionMouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
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Host speciesMouse
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SpecificityIn Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).
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Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Human
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Immunogen
Purified striated muscle actin from rabbit.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.097% Sodium azide
Constituent: 0.79% Tris HCl -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberAlpha Sr-1
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IsotypeIgM
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab28052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/50 - 1/100. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 42 kDa. |
Target
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FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similaritiesBelongs to the actin family.
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Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
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Database links
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 100009506 Rabbit
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
see all -
Alternative names
- a actin antibody
- ACTA antibody
- ACTA1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.com/ps/products/28/ab28052/Images/ab28052-229804-ab28052ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Nawazish Naqviab28052 staining Alpha Skeletal Muscle Actin in rabbit heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 24 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.com/ps/products/28/ab28052/Images/ab 28052 Abreview 10776.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.
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![Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.com/ps/products/28/ab28052/Images/ab 28052 Abreview 10777 logo.jpg)
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution
All lanes : 40 µg rat neonatal cardiac myocytes (whole cell lysate)
Secondary
All lanes : HRP polymer conjugated donkey anti-mouse IgG at 1/1000
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 second
This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.
Protocols
References
This product has been referenced in:
- Zhao S et al. High-pressure carbon dioxide pneumoperitoneum induces oxidative stress and mitochondria-associated apoptotic pathway in rabbit kidneys with severe hydronephrosis. Int J Mol Med 43:305-315 (2019). Read more (PubMed: 30431064) »
- Hehemann M et al. Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence. Neurourol Urodyn 37:2551-2559 (2018). Read more (PubMed: 30187971) »
