Recombinant
RabMAb

Recombinant Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

Overview

  • Product name

    Anti-Alpha Skeletal Muscle Actin antibody [EPR18430]
    See all Alpha Skeletal Muscle Actin primary antibodies
  • Description

    Rabbit monoclonal [EPR18430] to Alpha Skeletal Muscle Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Alpha Skeletal Muscle Actin aa 1-200. The exact sequence is proprietary.
    Database link: P68133

  • Positive control

    • WB: Human, mouse and rat skeletal muscle and heart lysates; Human bladder lysate. IHC-P: Mouse and rat skeletal muscle tissues.
  • General notes

    IHC is recommended for rat and mouse only.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/100.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin alpha cardiac muscle antibody
    • Actin, alpha skeletal muscle antibody
    • ACTS_HUMAN antibody
    • Alpha actin 1 antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • Cardiac muscle alpha actin 1 antibody
    • MPFD antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Weak cytoplasm staining in rat skeletal muscle is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • All lanes : Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate
    Lane 9 : Human brain lysate
    Lane 10 : Human heart lysate
    Lane 11 : Human kidney lysate
    Lane 12 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Weak cytoplasm staining in mouse skeletal muscle is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • All lanes : Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Mouse skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 5 seconds
  • Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution + Rat skeletal muscle lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution + Human bladder lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab184705 has not yet been referenced specifically in any publications.

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