Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Aorta)
Specification
Aorta
Fixative
10% neutral buffered formalin
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin-CaCl 0.0001% 30 min at 37*C
Permeabilization
No
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Concentration
4 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 0.3% Triton X-100 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Vector Labs VectaStain Elite ABC Kit (Universal)
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/50
Additional data
Additional Notes
Peroxidase Block: 10 min methanol/H2O2
ABC: 30 min Vectastain ABC (2%)
Peroxidase substrate: 5 min Vector NovaRED
Nuclear stain: 10 sec Vector Hematoxylin QS
Notes:
Excellent specificity on mouse tissue without need for mouse-on-mouse blocking kits.
Image: A = aSMA aorta @ 4 ug/ml, B = skin @ 4 ug/ml
ABC: 30 min Vectastain ABC (2%)
Peroxidase substrate: 5 min Vector NovaRED
Nuclear stain: 10 sec Vector Hematoxylin QS
Notes:
Excellent specificity on mouse tissue without need for mouse-on-mouse blocking kits.
Image: A = aSMA aorta @ 4 ug/ml, B = skin @ 4 ug/ml
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jan 24 2013