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Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Go to datasheet (ab7817)
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblast)
Permeabilization
No
Specification
Fibroblast
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: PBS
Dilution
1/400

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: FITC goat anti-mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Jan 17 2022