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Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Go to datasheet (ab7817)
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Glioblastoma multiforme)
Permeabilization
Yes - 0.25% TritonX-100
Specification
Glioblastoma multiforme
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Acetone + PFA

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: AffiniPure Goat Anti-Mouse IgG (Jackson ImmunoRese
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy2 ®
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Rouven Hoefflin

Verified customer

Submitted Nov 07 2022