Product nameAnti-alpha smooth muscle Actin antibody [1A4] (FITC)
See all alpha smooth muscle Actin primary antibodies
DescriptionMouse monoclonal [1A4] to alpha smooth muscle Actin (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-FoFr, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish
The N-terminal decapeptide of alpha-smooth muscle actin.
- In IF, this antibody gave a positive signal in SV40LT-SMC cells.
This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.
The antibody is conjugated with FITC at a ratio 6 Moles of Fluorescein to 1 Mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm, and maximum emission occurs at wavelenght 520 nm.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Primary antibody notesThis antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.
Light chain typekappa
- Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) (ab125057)
- Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 488) (ab184675)
- Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 594) (ab202368)
- Anti-alpha smooth muscle Actin antibody [1A4] (HRP) (ab203696)
- Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
- Anti-alpha smooth muscle Actin antibody [1A4], prediluted (ab8207)
Our Abpromise guarantee covers the use of ab8211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 17222057
ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/75. (as well as on cell smears).|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. PubMed: 17237513Use at an assay dependent dilution (from Pubmed:17237513).|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
ab8211 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8211 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).
This product has been referenced in:
- Zhou X et al. CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma. Oncol Lett 15:183-188 (2018). IF ; Human . Read more (PubMed: 29285191) »
- Lai YL et al. Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells. Redox Biol 15:51-61 (2018). Flow Cyt . Read more (PubMed: 29216542) »