Product nameAnti-alpha smooth muscle Actin antibody [1A4] (HRP)
See all alpha smooth muscle Actin primary antibodies
DescriptionMouse monoclonal [1A4] to alpha smooth muscle Actin (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Mammals, Baboon
Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).
Database link: P62736
- WB: HeLa, A431 and Jurkat whole cell lines. IHC-P: normal human colon tissue sections
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
Light chain typekappa
- Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) (ab125057)
- Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 488) (ab184675)
- Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 594) (ab202368)
- Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
- Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
- Anti-alpha smooth muscle Actin antibody [1A4] (FITC) (ab8211)
Our Abpromise guarantee covers the use of ab203696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
IHC image of alpha smooth muscle Actin staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab203696, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (HRP) (ab203696) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203696 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab203696 has not yet been referenced specifically in any publications.