Overview

  • Product name

    Anti-alpha smooth muscle Actin antibody [4A4]
    See all alpha smooth muscle Actin primary antibodies
  • Description

    Mouse monoclonal [4A4] to alpha smooth muscle Actin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide of Human alpha smooth muscle Actin (C-EYDEAGPSIVHRKCF) conjugated KLH

  • Positive control

    • Recombinant alpha smooth muscle Actin protein, Hela, A431, Jurkat, K562, HEK293, HepG2, NIH3T3, PC12 and COS7 cell lysates, HepG2 cells, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab119952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
  • All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS7 cell lysate

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of HepG2 cells using ab119952 at 1/200 (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (Red). DRAQ5 fluorescent DNA dye (blue).
  • Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).
  • ELISA, using ab119952 at a dilution of 1/10000.

References

This product has been referenced in:

  • Zhou B  et al. Rapamycin prevents thoracic aortic aneurysm and dissection in mice. J Vasc Surg 69:921-932.e3 (2019). Read more (PubMed: 30253896) »
  • Liu X  et al. Exosomes secreted by adipose-derived mesenchymal stem cells regulate type I collagen metabolism in fibroblasts from women with stress urinary incontinence. Stem Cell Res Ther 9:159 (2018). ICC/IF ; Human . Read more (PubMed: 29895333) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal problem mainly in immunohistochemical using tissue sections (mouse-on-mouse) due to endogenous mouse immunoglobins. In order to be able to reduce the risk of any high background signal, you may wish to select Fab fragment secondary antibodies - rather than full molecules.

I would recommend visiting this website; You may find some useful information on how to block high background signal:

https://www.abcam.com/index.html?pageconfig=resource&rid=11465

https://docs.abcam.com/pdf/protocols/Mouse-on-Mouse-Protocol.pdf

Read More

Answer

The histogram showed in the datasheet was obtained using 4% paraformaldehyde as fixative agent, and 0.1% Triton-X100 and 0.5% BSA in 1X PBS to permeabilise.

Read More

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