Anti-alpha smooth muscle Actin antibody (ab15734)

Overview

  • Product name
    Anti-alpha smooth muscle Actin antibody
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Rabbit polyclonal to alpha smooth muscle Actin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC (PFA fixed), IHC-FoFr, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Human, Zebrafish
    Predicted to work with: Rabbit, Chicken, Cow
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human alpha smooth muscle Actin.

    (Peptide available as ab16102.)

  • Positive control
    • Hela Whole Cell Lysate A431 Whole Cell lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Kidney (Mouse) Tissue Lysate Ovary (Mouse) Tissue Lysate - normal tissue Brain (Rat) Tissue Lysate - normal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab15734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/200.
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Images

  • Ab15734 detecting Alpha Smoth Muscle Actin in Rat Heart. The tissue were fixed (animal Perfused fixed) with 4% PFA and Later postfixed overnight i the same fixitive. The tissue is cryoprotected in 30% sucrose and cut uisng a cryostat

    See Abreview

  • All lanes : Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Kidney (Mouse) Tissue Lysate
    Lane 5 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 6 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 41 kDa (why is the actual band size different from the predicted?)

  • ab15734 staining alpha smooth muscle Actin in Horse trachealis muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in ethanol prior to blocking with 10% BSA for 1 hour at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An EXPOSE Polyvalent HRP/DAB detection kit (ab80436) was used for detection.

    See Abreview

  • Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/ml + Recombinant Human alpha smooth muscle Actin protein (ab114148) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa


    Exposure time: 20 seconds


    Ab15734 recognizes the full length tagged recombinant alpha small muscle Actin protein (ab114148) which has an expected molecular weight of 67 kDa.
  • All lanes : Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa whole cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml
    Lane 4 : A431 cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat-anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa

References

This product has been referenced in:
  • Yan C  et al. Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin avß5 in Zebrafish. Neoplasia 20:533-542 (2018). Read more (PubMed: 29649779) »
  • Ono E  et al. Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment. PLoS One 13:e0195960 (2018). Read more (PubMed: 29677207) »

See all 21 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Cell (peritoneal mesothelial cells)
Specification
peritoneal mesothelial cells
Permeabilization
No
Fixative
Acetone:methanol=1:1
Username

Abcam user community

Verified customer

Submitted Nov 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Sample
Mouse Tissue lysate - whole (Heart-day 0 and day 7 post MI LV)
Specification
Heart-day 0 and day 7 post MI LV
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Merry Lindsey

Verified customer

Submitted Sep 20 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Uterine smooth muscle (myometrium))
Loading amount
100 µg
Specification
Uterine smooth muscle (myometrium)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Feb 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal human prostate)
Specification
Normal human prostate
Fixative
Neutral buffered formalin
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Jan 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Trachealis muscle)
Specification
Trachealis muscle
Fixative
Ethanol
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted May 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa and HCT116)
Loading amount
40 µg
Specification
HeLa and HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Mar 06 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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