Overview

  • Product name

    Anti-alpha smooth muscle Actin antibody
    See all alpha smooth muscle Actin primary antibodies
  • Description

    Goat polyclonal to alpha smooth muscle Actin
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human alpha smooth muscle Actin aa 3-12 (N terminal).
    Sequence:

    EEEDSTALVC


    Database link: P62736

  • Positive control

    • WB: HeLa and NIH3T3 cell lysates, Mouse and Human heart tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab21027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:4,000.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Images

  • All lanes : Anti-alpha smooth muscle Actin antibody (ab21027) at 0.1 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Mouse heart lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa

  • Lane 1 : Anti-alpha smooth muscle Actin antibody (ab21027) at 0.1 µg/ml
    Lane 2 : Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa

References

This product has been referenced in:

  • Sun C  et al. Meis2 represses the osteoblastic transdifferentiation of aortic valve interstitial cells through the Notch1/Twist1 pathway. Biochem Biophys Res Commun 509:455-461 (2019). Read more (PubMed: 30594396) »
  • Luo H  et al. The Role of Tauroursodeoxycholic Acid on Dedifferentiation of Vascular Smooth Muscle Cells by Modulation of Endoplasmic Reticulum Stress and as an Oral Drug Inhibiting In-Stent Restenosis. Cardiovasc Drugs Ther 33:25-33 (2019). Read more (PubMed: 30663009) »
See all 54 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

Submitted Aug 01 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Heart
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

Submitted Jul 31 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100X Citrate Buffer pH 6.0 (ab94674)
Permeabilization
Yes - 0.05% tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

Submitted Jun 17 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Epithelial (hTert-RPE1))
Permeabilization
No
Specification
Epithelial (hTert-RPE1)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted May 23 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung, smooth muscle cell)
Permeabilization
Yes - sodium citrate buffer
Specification
lung, smooth muscle cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (femoral artery)
Permeabilization
Yes - 0.1% Triton-X
Specification
femoral artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MSC)
Permeabilization
Yes - Triton 1%
Specification
MSC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 08 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - Triton 1%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 02 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 20 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Sample
Human Tissue sections (Mammary fat pad)
Specification
Mammary fat pad
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 24 2014

1-10 of 15 Abreviews or Q&A

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