Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to alpha smooth muscle Actin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Human
Product nameAnti-alpha smooth muscle Actin antibody
See all alpha smooth muscle Actin primary antibodies
DescriptionRabbit monoclonal to alpha smooth muscle Actin
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human alpha smooth muscle Actin aa 1-50. The exact sequence is proprietary. NP_001135417.1
Database link: P62736
- IHC-P: Human breast tissue. Human colon carcinoma tissue. ICC: IMR-90 cells. WB: IMR-90 and NIH/3T3 whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab265588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
All lanes : Anti-alpha smooth muscle Actin antibody (ab265588) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate at 50 µg
Lane 2 : A549 whole cell lysate at 50 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : IMR-90 whole cell lysate at 10 µg
Lane 5 : HepG2 whole cell lysate at 50 µg
Lane 6 : LNCaP whole cell lysate at 50 µg
Lane 7 : RKO whole cell lysate at 50 µg
Lane 8 : K562 whole cell lysate at 50 µg
Lane 9 : NIH/3T3 whole cell lysate at 50 µg
Lane 10 : TCMK whole cell lysate at 50 µg
Predicted band size: 42 kDa
Exposure time: 30 seconds
Cells prepared using NETN lysis buffer.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
Immunocytochemical analysis of formalin-fixed, paraffin-embedded IMR-90 cells labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265588 has not yet been referenced specifically in any publications.