Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (aorta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer pH 6
Permeabilization
Yes - Triton X-100, 0.2%
Specification
aorta
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA/PBS
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa 555 Goat anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Anti-alpha smooth muscle Actin (Red) counter-stained with DAPI (Blue).
Abcam user community
Verified customer
Submitted Jul 25 2019